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STOCK Tg(Nefh-cre)12Kul/J
Cat. No.: CEMM-07250150
Mice harboring this mNF-H-cre transgene have nuclear localized-Cre recombinase expression directed primarily to cortex and hippocampus neurons coinciding the late stages of brain development by the mouse neurofilament-H gene promoter. These mice may be useful in generating conditional knockouts for neurobiological, neurodevelopmental, neuromuscular, amyotrophic lateral sclerosis (ALS or Lou Gehrig's disease), or aging studies involving the neurons of the brain and spinal cord.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Nefh-cre)12Kul
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| Allele Name |
transgene insertion 12
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Tg(Nefh-cre)12Kul
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| Gene Name |
transgene insertion 12
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| Chromosome |
UN
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Expression of Cre recombinase is driven in neurons of the brain and spinal cord during late development (around E18.5).
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| Strain of Origin |
FVB/N
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| Molecular Note |
An 8.5 kb promoter fragment of mouse Nefh, containing the 5' UTR and 40 bp of non-coding sequence of exon 1 ending before the start codon, was adjoined to sequence encoding Cre recombinase with an SV40 nuclear localization signal. An SV40 polyadenylation signal was also included in the construct. Expression of Cre recombinase is driven in neurons of the brain and spinal cord during late development (around E18.5). Line is best described and is representative of the other lines.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Noncarrier
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| Breeding Considerations |
When maintaining a live colony, hemizygous mice may be bred together, to non-carrier (wildtype) siblings, or to FVB/NJ inbred mice. The donating investigator reports that homozygous mice develop a neurological phenotype with seizures and become infertile and moribund.
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For Research Use Only.
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