C57BL/6J-Tg(ACTB-NOTCH1)1Shn/J

Cat. No.: CEMM-07250053

Common Name: CALSL-NICD

These mutant mice contain a "Lox-STOP-Lox" cassette that prevents expression of the human NOTCH1 transgene until excised by Cre-recombinase. This strain may be useful for generating conditional mutations in applications related to the study of notch signaling during apoptotic cell death.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(ACTB-NOTCH1)1Shn show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Inserted expressed sequence; Humanized sequence show more close
Gene Symbol	Tg(ACTB-NOTCH1)1Shn show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	NOTCH1, notch 1, human show more close
MGI Accession ID	3044583 show more close
Strain of Origin	C57BL/6J show more close
Molecular Note	This transgene consists of the chicken beta-actin promoter followed by a loxP flanked stop, the human NOTCH1 intracellular domain (amino acids 1762-2304), and an SV40 late polyadenylation signal. Cre mediated recombination removes the stop and allows expression of the NOTCH1 gene. Only single site insertions were used to generate mice. This line carries 10 - 20 copies of the transgene and protein is detectable by western blot analysis after Cre mediated recombination. show more close

HUSBANDRY
Suggested Controls	C57BL/6J Noncarrier show more close
Breeding Considerations	When maintaining a live colony, hemizygotes are bred to wildtype siblings or to C57BL/6J inbred mice. show more close

For Research Use Only.