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Brainbow Service
Brainbow is a genetic technology used for labeling individual cells to visualize and differentiate them. It has the potential to greatly advance our understanding of complex biological systems and could lead to the development of new treatments for a wide range of diseases. Creative Biogene offer brainbow-based services to our global customers for assisting them making better breakthrough in their research area.
Introduction into Brainbow
The brainbow technology is based on site-specific recombinase system, resulting in the expression of multiple fluorescent proteins in a single cell. It is a genetic technology used to label and visualize individual cells with in tissues or organs, especially in the nervous system. This technology was first applied in mice, then adapted in other model organisms such as zebrafish, fruit flies and C. elegans.
The Brainbow technique has been further developed and optimized since its initial creation. Variants of the technique include Confetti, allowing for clonal labeling, and Brainbow 3.0, 3.1 and 3.2, which allow for greater spectral diversity and improved expression levels.
Strategies of brainbow (Cai, Dawen et al., 2013)
Brainbow works by introducing multiple copies of a gene encoding for fluorescent proteins, such as green, red, and blue, into a group of cells. The copies of the gene are randomly recombined using site-specific recombination enzymes, resulting in each cell expressing a unique combination of fluorescent proteins. This allows researchers to differentiate individual cells based on their unique color signature.
Advantages of Brainbow
Compared with other fate mapping technologies, brainbow technology has several advantages over them. Followings are some of the advantages:
| Multicolor Labeling | Unlike other techniques that typically label cells with only one color, Brainbow allows for the expression of multiple fluorescent proteins in a single cell. This enables researchers to visualize and differentiate between individual cells within a complex tissue with greater precision. |
| Clonal Labeling | Brainbow variants, such as Confetti, allow for clonal labeling of cells, meaning that cells that arise from a single progenitor cell can be labeled with the same fluorescent protein combination. This allows for the study of cell lineage and development. |
| High Flexibility | The Brainbow is compatible with a wide range of genetic tools and techniques, allowing researchers to tailor it to their specific needs. |
| High-resolution imaging | The Brainbow is compatible with high-resolution imaging techniques, such as confocal microscopy, which allows for the visualization of individual cells and their processes in great detail. |
| Wide-range Applications | The Brainbow has been applied in a wide range of fields, including neuroscience, developmental biology, and regenerative medicine, making it a versatile tool for studying various biological systems. |
Our Service
The high resolution and multiplexing capabilities of the Brainbow technology make it a valuable tool for drug discovery and could lead to the development of new treatments for a wide range of diseases. Creative Biogene offers Brainbow-based services included but not limited as follows.
- Cell identification service
Base on brainbow technology, Creative Biogene offers cell identification service to study cell genesis within complex tissues, such as the brain. - Cell tracing service
Creative Biogene offers cell tracing services with the brainbow technology for better understanding on how different cell types are formed. - Circuit mapping service
We provide circuit mapping service based on the brainbow technology, which can tell the connections between different cells, such as neurons in the brain. - Disease modeling service
We provide disease modeling service based on our brainbow technology, such as Alzheimer's disease and Parkinson's disease, to study the progression of these diseases and test potential treatments. - Regenerative medicine development service
We provide regenerative medicine development service via brainbow, which put insight on the process of tissue regeneration, such as the regeneration of the spinal cord after injury.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Cai, Dawen et al. "Improved tools for the Brainbow toolbox." Nature methods vol. 10,6 (2013): 540-7.