Technology
Online Inquiry
Recombinase Mediated Cassette Exchange (RMCE)
Recombinase-mediated cassette exchange (RMCE) is a genetic engineering technique used to introduce specific changes into the genome of an organism. The method is based on the use of site-specific recombinases, enzymes that can recognize specific DNA sequences and catalyze the exchange of genetic material between two sites.
What Is RMCE?
In RMCE, the desired genetic change is incorporated into a recombination substrate, which is a DNA molecule that contains the desired modification and a recognition site for the site-specific recombinase. The recombination substrate is introduced into the target cell, and the site-specific recombinase is used to integrate the substrate into the genome of the cell, replacing the target sequence with the desired modification.
RMCE has many applications in genetic engineering, particularly in the creation of animal models for research. It allows for the introduction of precise genetic modifications without disrupting the endogenous genetic environment, and can be used to introduce multiple modifications in a single animal. RMCE can also be used to replace a disease-causing gene with a corrected version, providing a potential cure for genetic disorders.
-1.jpg)
Other RMCE Technologies
- Dual RMCE
Dual RMCE (dRMCE) takes advantage of the fact that most conditional alleles encode a selection cassette flanked by FRT sites, except for the loxP site that flanks functionally related exons. The FRT flanking selection cassette is usually located outside the loxP flanking region, which makes these alleles directly compatible with dRMCE. This allows for the simultaneous exchange of two separate genomic regions, making it possible to introduce or delete two different genes or genetic elements at the same time. This is particularly useful for studying the interactions between genes and for creating more complex genetic modifications. - Multiplexing RMCE
In Multiplexing RMCE, multiple site-specific recombinases are used to perform multiple RMCE reactions in parallel. This allows for the simultaneous exchange of multiple genomic regions, making it possible to introduce or delete multiple genes or genetic elements at the same time. This is particularly useful for studying the interactions between genes and for creating more complex genetic modifications.
Applications of RMCE
RMCE has several advantages over traditional genetic engineering techniques, such as being a precise, efficient, and highly specific method for introducing genetic changes into the genome of a cell. RMCE has been used in a wide range of applications, including the generation of transgenic animals, the production of human cell lines for research, and the generation of genetically modified crops. Followings are applications of RMCE:
- Model organism engineering: RMCE can be used to modify the genomes of model organisms, such as mice, to study the functions of specific genes in a controlled and controlled manner.
- Functional genomics: RMCE can be used to study the functions of specific genes by introducing mutations into the genome or by expressing genes in a specific tissue or cell type.
- Inducible gene activation: RMCE can be used to activate specific genes that are normally silent in a particular tissue or cell type, allowing researchers to study their functions.
- Gene repression: RMCE can be used to repress specific genes in a particular tissue or cell type, allowing researchers to study the effects of gene repression on development and disease.
- Conditional gene knock-out: RMCE can be used to delete specific genes from the genome, allowing researchers to study the functions of those genes by observing the changes in phenotype.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.