SSR-mediated Fate Mapping Service

Site-specific recombinase (SSR)-based fate mapping is a method used to trace the lineage of cells in a developing organism. It is a genetic technique that uses a site-specific recombination system, to track the descendants of a specific cell or group of cells. Creative Biogene offers SSR-mediated fate mapping service.

Introduction into Fate Mapping Using SSR System

To perform fate mapping using the SSR system, researchers first engineer a transgenic mouse in which a specific cell type of interest expresses the Cre recombinase. This is typically done using a promoter that is specific to the cell type of interest. Then, the researchers introduce a DNA construct into the mouse that contains two loxP sites flanking a gene of interest. This gene is often a reporter gene, such as green fluorescent protein (GFP), that allows the researchers to visualize the cells that have undergone recombination.

SSR-based strategies to fate mapping (Meinke, Gretchen et al. 2016)

Once the transgenic mouse is generated and the DNA construct is introduced, the researchers can track the lineage of the cells expressing the Cre recombinase by observing the expression of the GFP reporter gene. If a cell expressing the Cre recombinase undergoes a recombination event, the loxP sites flanking the GFP gene will be deleted and the gene will be activated, resulting in the expression of GFP in that cell and its descendants. By tracking the expression of GFP over time, the researchers can determine the developmental trajectory and fate of the cells expressing the Cre recombinase.

Applications of Fate Mapping Using SSR System

One powerful approach for fate mapping is to use site-specific recombinase systems, such as Cre-loxP or FLP-FRT, to induce permanent genetic changes in specific cells or tissues. There are many potential applications of fate mapping using site-specific recombinase systems, including:

• Neural lineages: SSR technology has been used to trace the developmental trajectory of specific neural cell types, such as neurons and glia, in the developing brain. For example, researchers have used the SSR system to study the lineage and differentiation of neural stem cells and the formation of specific brain regions.

Brainbowvia site-specific recombinase system (Meinke, Gretchen et al. 2016)

• Cardiac development: This technologyhas been used to study the development and function of the heart, including the fate of specific cardiac cell types, such as cardiomyocytes and vascular smooth muscle cells. Researchers have used this technology to trace the lineage of cardiac progenitor cells and study the formation of the heart chambers and blood vessels.
• Hematopoietic lineages: SSR has been used to study the development of the hematopoietic system, which is responsible for producing blood cells. Researchers have used this technology to trace the lineage of hematopoietic stem cells and study the formation of specific blood cell types, such as red blood cells, white blood cells, and platelets.
• Liver development: SSR has been used to study the development of the liver and the differentiation of liver progenitor cells into specific liver cell types, such as hepatocytes and bile duct cells. Researchers have used this technology to study the formation of the liver and the regulation of liver function.
• Pancreatic development: SSR has been used to study the development of the pancreas and the differentiation of pancreatic progenitor cells into specific cell types, such as alpha and beta cells. Researchers have used this technology to study the formation of the pancreas and the regulation of insulin secretion.

Our Service

Creative Biogene offers fate mapping service based on site-specific recombinase system. Our services are included but not limited as follows

Why Choose Us

Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEdit^TM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.

Reference

1. Meinke, Gretchen et al. "Cre Recombinase and Other Tyrosine Recombinases." Chemical reviews vol. 116,20 (2016): 12785-12820.

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• Inducible Fate Mapping Service
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