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Services Based on Site-specific Recombinase
Site-specific recombinase can recognize and bind to specific DNA sequences and catalyze the recombination between the specific sites and result in insertion, deletion or exchange of the DNA fragments between the specific sites. Creative Biogene offer site-specific recombinase technology-based services to our global customers for assisting them making better breakthrough in their research area.
Introduction into Site-specific Recombinase System
Site-specific recombinase systems are a group of molecular tools used to manipulate DNA in a specific and controlled manner. These systems rely on the action of recombinases, which catalyze the site-specific recombination of two DNA sequences that share a short region of homology. Based on the direction of specific sites, DNA fragment can be deleted, inserted, inverted, translocated or cassette exchanged.
The site-specific recombinase systems are widely used in genetic engineering and biotechnology, especially in the development of conditional knockout animal models, gene therapy, and synthetic biology. The most popular site-specific recombinase systems are Cre/loxP, Flp/FRT, and Dre/rox. Following is the comparison of the most widely used SSR systems.
| SSR System | Advantages | Disadvantages |
|---|---|---|
| Cre/loxP |
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| Flp/FRT |
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| Dre/rox |
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Applications of Site-specific Recombinase System
Due to the specify, precision, versatility and efficiency of site-specific recombinase, it has become an essential tool in molecular biology, genetics, and biotechnology. Its ability to precisely manipulate DNA enable researchers to launch gene functional study easily, as well as the developmental biology study. Followings are main applications of site-specific recombinase.
- Conditional Gene Knockout
Site-specific recombinase systems can be used to conditionally delete or knock out genes in specific tissues or at specific developmental stages. This enables researchers to study the role of a gene in a specific context, which is essential for understanding its function. - Inducible Gene Expression
Site-specific recombinase systems can be used to activate or inactivate transgenes in a specific tissue or at a specific developmental stage. This allows researchers to study the effects of gene expression in a controlled manner. - Genome Engineering
Site-specific recombinase systems can be used to precisely modify the genome, enabling the insertion, deletion, or inversion of specific DNA sequences. This is useful for creating animal models of human diseases, studying gene function, and developing gene therapies. - Cell Lineage Tracing
Site-specific recombinase systems can be used to label cells with a specific marker, allowing researchers to track their lineage and fate. This is useful for understanding how tissues develop and how diseases progress. - Synthetic Biology
Site-specific recombinase systems can be used to create genetic circuits and networks for synthetic biology applications. This allows researchers to design and engineer biological systems with specific functions, such as biosensors and bioremediation systems.
Our Service
With edge-cutting technologies and equipment and years of experience, Creative Biogene offers services based on our optimized site-specific recombinase technologies. Our services are included but not limited as follows, please view the following pages for detailed information.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.