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Customized Xenopus Models
Xenopus laevis and Xenopus tropicalis, commonly known as African clawed frogs, are two species of amphibians that are widely used as model organisms in biological research. Creative Biogene offer customized zebrafish modeling services to our global customers for assisting them making better breakthrough in their research area.
Introduction into Xenopus Models Generated by T-SSR System
One of the advantages of using Xenopus as a model organism for site-specific recombinase technology is their external fertilization and transparent embryos, which allow for easy access to specific cells and tissues for targeted gene modifications. In addition, the large size of Xenopus embryos and their ability to survive without a functional cardiovascular system during early development make them suitable for genetic manipulations that may not be feasible in other model organisms.
Conditional labeling in Prrx1:CreER frog lines (Lin, Tzi-Yang et al., 2022)
In Xenopus, researchers have used Cre/loxP technology to generate human disease models, such as cancer and neurological disorders. In these models, the Cre recombinase is expressed under the control of a tissue-specific promoter, allowing for conditional gene knockout or overexpression in that tissue or cell type. These models have been valuable in elucidating the molecular mechanisms underlying these diseases.
Another example is the use of T-SSR technology to study the role of the homeobox transcription factor Otx2 in eye development. By introducing loxP sites into the Otx2 gene and crossing these frogs with a transgenic line expressing Cre recombinase under the control of an eye-specific promoter, researchers were able to selectively delete Otx2 in the developing eye and observe its effects on eye development.
Xenopus is a popular model organism in developmental biology due to its unique biological characteristics, such as external fertilization, rapid development, large and easily accessible eggs, and well-characterized developmental stages. Followings are some of the advantages of Xenopus.
| Advantages of Xenopus as Model Organism | |
|---|---|
| Large eggs and embryos | Xenopus embryos are large and easy to manipulate, allowing researchers to study developmental processes at the cellular and molecular level. |
| External development | Xenopus embryos develop externally, making it easy to observe and manipulate them without damaging the embryo. |
| Amphibian biology | Xenopus is an amphibian, and its biology is more similar to that of mammals than other model organisms like fruit flies or nematodes. |
| Easy to maintain | Xenopus is relatively easy to maintain in the laboratory, and can be housed in standard aquaria. |
| Short generation time | Xenopus has a short generation time, allowing researchers to produce large numbers of offspring in a relatively short period of time. |
| Regeneration | Xenopus is capable of regenerating tissues and organs, making it a useful model organism for studying tissue repair and regeneration. |
Applications of Our Customized Xenopus Models
- Developmental biology: By knocking out genes at specific stages of development, researchers can observe the effects of gene loss on various developmental processes.
- Regeneration: Conditional knockout technology can be used to investigate the genes and signaling pathways that are involved in regeneration, providing insights into how to promote regeneration in other organisms.
- Cancer research: Researchers can use cKO Xenopus to knock out tumor suppressor genes and observe the resulting effects on tumor growth.
- Neuroscience: By knocking out genes in specific regions of the brain or at specific developmental stages, researchers can investigate how genes contribute to neural development, behavior, and disease.
Our Service
Creative Biogene offers Xenopus model generation service based on site-specific recombinase. Please view the following pages to check out the detailed information of our services.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Lin, Tzi-Yang et al. Inducible and tissue-specific cell labeling in Cre-ERT2 transgenic Xenopus lines. Development, growth & differentiation vol. 64,5 (2022): 243-253.