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Customized Animal Models
Generating animal models with site-specific recombinase systems is a widely used technique in genetic engineering, and it allows for precise manipulation of the genome. Site-specific recombinases such as Cre-loxP and Flp-FRT systems have been used to generate knockout, knockin, and conditional knockout models, as well as for gene tagging and cell lineage tracing. Creative Biogene offers customized animal model generation services via site-specific recombinase-based technologies.
Introduction into Animal Models Generated by T-SSR System
In animal modeling, the site-specific recombinase system is typically used to generate conditional knockout or knockin animal models. Conditional knockout animal models are generated by inserting specific sites around a specific gene, which allows for tissue-specific deletion of the gene of interest when crossed with a recombinase-expressing model.
The site-specific recombinase system has been widely used for generating conditional KO mouse model. This enables researchers to study the role of specific genes in development and disease in a spatially and temporally controlled manner. Similarly, knockin mice can be generated by inserting a new gene or mutation at a specific locus using loxP sites and Cre recombinase.
Conditional KO mouse using the Cre-loxP (Kovilakath, Anna et al., 2018)
As other example, in zebrafish, the Cre/loxP system has been used to generate tissue-specific knockout and knockin lines to study gene function during development. In Drosophila, the system has been used to study neural circuits and behavior by generating mutant lines with specific mutations in neurons.
In rats, the Cre/loxP system has been used to study cardiovascular and metabolic diseases. Similarly, in rabbits, the system has been used to generate animal models for studying retinal degeneration and cardiovascular diseases.
Workflow for Customized Animal Modeling
Our customized animal modeling service is based on site-specific recombinase system, in which, the enzymes recognize and bind to specific DNA sequences, and then catalyze a recombination event between two target sites to excise, invert, or relocate DNA sequences. There are several types of site-specific recombinases, but the most commonly used ones are the Cre/loxP system and the Flp/FRT system. The workflow of our service is as follows:
Design and construction of genetic constructs: This involves designing and constructing the genetic constructs that will be used to introduce the site-specific recombinase into the animal model.
Generation of transgenic animals: The genetic constructs are introduced into the animal model using methods such as pronuclear injection, embryonic stem cell transfection, or viral transduction.
Breeding of transgenic animals: The transgenic animals are bred to generate a stable line of animals carrying the recombinase.
Induction of site-specific recombination: The site-specific recombinase is activated in the animal model using various methods such as administration of tamoxifen or doxycycline.
Analysis of the animal model: The animal model is analyzed to assess the effects of the site-specific recombination, such as conditional knockout or tissue-specific gene expression.
Iterative optimization: The animal model can be further optimized through iterative rounds of design, generation, and analysis to improve the specificity and efficiency of the site-specific recombinase system.
Our Service
Creative Biogene offers animal models generation service based on site-specific recombinase. With the optimized T-SSR-based technologies, we offer model generation service in animals including but not limited as follows:
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Kovilakath, Anna et al. "In Vitro Erythroid Differentiation and Lentiviral Knockdown in Human CD34+ Cells from Umbilical Cord Blood." Methods in molecular biology (Clifton, N.J.) vol. 1698 (2018): 259-274.