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Customized Cell Lines
Cell lines are a valuable tool for studying basic cellular processes, as well as for drug discovery, toxicology, and disease modeling. Their ease of use, genetic manipulability, homogeneity, safety, and high-throughput capabilities make them a versatile and powerful system for a wide range of applications. Creative Biogene offers customized cell lines modeling services based on our edge-cutting site-specific recombinase-based technologies to meet our global customers' requirements.
Introduction into Mouse Models Generated by T-SSR System
To use site-specific recombinase for genetic engineering in cell lines, researchers typically introduce a plasmid or viral vector that expresses recombinase into the cells of interest. The vector also carries a DNA construct that contains specific sites flanking the target DNA sequence. When recombinase is expressed in the cells, it catalyzes the recombination between the loxP sites, resulting in the removal, inversion, or translocation of the target DNA sequence.
Process of generating inducible KO hPSC cell line (Chen, Yuejun et al. 2015)
One of the most common applications of site-specific recombinase technology is to generate KO cell lines, in which specific genes are disrupted or deleted from the genome. This is achieved by introducing loxP sites flanking the target gene, and expressing Cre or Flp recombinase to catalyze the excision or inversion of the gene of interest. This approach can be used to study the function of individual genes or genetic pathways in a cellular context. For conditional KO cell lines, the specific sites are introduced around the target gene and the recombinase is activated by switch. This approach can be used to study the role of genes in different cellular contexts or disease models.
Site-specific recombinase technology can also be used to insert or remove specific DNA sequences, or to activate or repress gene expression by manipulating regulatory elements. This approach allows researchers to study the effects of altered gene expression on cellular function or disease progression.
Cre Reporter Cell Lines
The most widely used one is Cre reporter cell lines, which is genetically engineered to express a reporter gene in response to Cre recombinase activity. The purpose of a Cre reporter cell line is to visualize or detect cells that have undergone Cre-mediated recombination, allowing for the identification and characterization of cells with specific gene expression patterns or lineage histories.
Strategy to generate lineage-specific Cre reporter hPSC lines (Bao, Xiaoping et al. 2019)
In general, Cre reporter cell lines are used in conjunction with Cre recombinase-expressing cells or animals to trace cell lineages, study gene expression patterns, or validate the efficiency and specificity of Cre-mediated recombination. By visualizing the expression of the reporter gene, researchers can identify and isolate cells that have undergone Cre-mediated recombination, allowing for further characterization or manipulation of these cells.
Our Service
Creative Biogene offer customized cell line development service based on our optimized T-SSR system, with the advanced technologies and years of experiences. Our customized cell line development services are included but not limited as follows:
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
References
- Chen, Yuejun et al. "Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9." Cell stem cell vol. 17,2 (2015): 233-44.
- Bao, Xiaoping et al. Gene Editing to Generate Versatile Human Pluripotent Stem Cell Reporter Lines for Analysis of Differentiation and Lineage Tracing. Stem cells (Dayton, Ohio) vol. 37,12 (2019): 1556-1566.