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Intersectional Fate Mapping Service
Intersectional Fate Mapping is a technique used in molecular biology and neuroscience to label and trace the fate of specific cell populations in complex tissues or organisms. Creative Biogene offer intersectional fate mapping services to our global customers for assisting them making better breakthrough in their research area.
Introduction into Intersectional Fate Mapping
Intersectional fate mapping is a technique used to trace the lineage and fate of cells during development, using two or more site-specific recombinases to activate gene expression in specific cell populations. This technique allows for the precise and temporal control of gene expression in specific subsets of cells, and is particularly useful for studying complex biological processes such as organogenesis and tissue patterning.
Process of Intersectional Fate Mapping with Cre and Flp reporter (Poulin, Jean-François et al., 2020)
In intersectional fate mapping, two different site-specific recombinases are used to activate gene expression in specific subsets of cells. The first recombinase, often Cre, is activated by a specific promoter, resulting in the expression of a recombinase-dependent marker gene such as GFP. The second recombinase, often Flp, is activated by a different promoter, resulting in the expression of a second recombinase-dependent marker gene such as RFP. When these two recombinases are expressed in overlapping cell populations, the resulting double-positive cells express both marker genes, allowing for the lineage tracing of these cells during development.
Applications of Intersectional Fate Mapping
Intersectional fate mapping technology has several advantages over traditional fate mapping technology, which can overcome the limitation of single recombinase system, such as the incomplete labeling and loss of expression. Followings are some applications of intersectional mapping:
- Precise and temporal control of gene expression: Intersectional fate mapping allows for the precise and temporal control of gene expression in specific subsets of cells, which is particularly useful for studying complex biological processes such as organogenesis and tissue patterning.
- Lineage tracing: By using two or more site-specific recombinases, intersectional fate mapping allows for more complete lineage tracing of cells during development, overcoming some of the limitations of traditional fate mapping techniques.
- Study of complex tissue and organ systems: The use of multiple recombinases in intersectional fate mapping enables the study of lineage and fate in more complex tissue and organ systems, such as the nervous system, heart and vasculature, and limb buds.
- Study of disease processes and therapies: Intersectional fate mapping has been used to study disease processes such as cancer, and to develop new therapies for genetic disorders such as muscular dystrophy.
Our Service
Creative Biogene offers mouse models generation service based on site-specific recombinase
- Cell lineage analysis service
Intersectional fate mapping can be used to map the lineage of specific cell populations during embryonic development or tissue regeneration. - Neuroanatomy service
Intersectional fate mapping can be used to study the development and organization of the nervous system, including the formation of neural circuits and the migration of neuronal cells. - Disease modeling service
Intersectional fate mapping can be used to generate disease models that mimic the cellular and molecular changes associated with specific diseases, such as cancer or neurodegenerative disorders. - Cell fate determination service
Intersectional fate mapping can be used to identify the factors that determine the fate of specific cell populations, such as stem cells or progenitor cells. - Drug discovery service
Intersectional fate mapping can be used to identify novel targets for drug discovery, by studying the behavior of specific cell populations in response to pharmacological interventions.
Why Choose Us
One-stop Service
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Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Poulin, Jean-François et al. "PRISM: A Progenitor-Restricted Intersectional Fate Mapping Approach Redefines Forebrain Lineages." Developmental cell vol. 53,6 (2020): 740-753.e3.