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Customized Zebrafish Models
The use of site-specific recombinase technology in zebrafish has provided a powerful tool for studying gene function and developmental processes, and has the potential to aid in the development of new therapies for human disease. Creative Biogene offer customized zebrafish modeling services to our global customers for assisting them making better breakthrough in their research area.
Introduction into Zebrafish Models Generated by T-SSR System
Generating genetically modified zebrafish using site-specific recombinase technology requires careful design of the targeting construct, microinjection into zebrafish embryos, breeding with driver lines, screening for recombination events, and characterization of the resulting transgenic lines. This approach offers a powerful tool for studying gene function and developmental processes in zebrafish.
Cre-mediated recombination fluorescence change event in zebrafish (Yen, Shuo-Ting et al. 2020)
Zebrafish are relatively easy to maintain in the laboratory, requiring simple equipment and low-cost feed. They are commonly housed in tanks with a temperature-controlled environment and a photoperiod to simulate day and night. Researchers can manipulate the genetics of zebrafish easily, which allows the introduction of exogenous genes into the zebrafish genome.
Overall, zebrafish offer a valuable tool for studying genetic and developmental processes, modeling human diseases, and testing potential drug candidates, and their use in biomedical research is likely to continue to expand in the coming years.
Advantages of Zebrafish as Model Organism
Zebrafish is a widely used model organism in biological research due to its unique features such as rapid development, transparency of embryos, small size, and high fecundity. Zebrafish have a relatively small genome, which has been fully sequenced, and share a high degree of genetic similarity with humans. Followings are some of the advantages of zebrafish as model organism.
| External development | Zebrafish embryos are transparent and develop externally, which makes it easy to observe and manipulate them under the microscope without damaging the embryo. |
| High fecundity | Zebrafish can lay hundreds of eggs at a time, making it easy to generate large numbers of offspring for experiments. |
| Short generation time | Zebrafish have a short generation time of around three to four months, which allows researchers to produce multiple generations of fish in a relatively short amount of time. |
| Regenerative capacity | Zebrafish are capable of regenerating several organs, such as the heart, spinal cord, and fins, making them useful for studying tissue repair and regeneration. |
| Similarity to human biology | Zebrafish share many similarities with humans in terms of their genetics, developmental biology, and organ function, making them a useful tool for studying human diseases and drug development. |
| Easy to maintain | Zebrafish are easy to maintain in the laboratory, requiring only simple equipment and low-cost feed. |
Our Service
Creative Biogene offers zebrafish model generation service based on site-specific recombinase. For providing multiple solutions to the biological problems, we have developed many well-established T-SSR-based technologies, such as split-Cre, light-activatable T-SSR and many others. Our zebrafish model services include:
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Yen, Shuo-Ting et al. CreLite: An optogenetically controlled Cre/loxP system using red light. Developmental dynamics : an official publication of the American Association of Anatomists vol. 249,11 (2020): 1394-1403.