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Inducible Fate Mapping Service
Inducible fate mapping is a very powerful technology that provide temporal and spatial control over gene expression, which allows for precise and specific analysis of biological questions. Creative Biogene offers inducible fate mapping services to our global customers for assisting them making greater breakthrough in a wide range of applications in basic research, disease research, and therapeutic development.
Introduction into Inducible Fate Mapping
Inducible fate mapping is a technique used to trace the lineage and fate of cells during development or in adult organisms, using an inducible system to activate gene expression in specific cell populations. This technique allows for the temporal control of gene expression in specific subsets of cells, and is particularly useful for studying the dynamics of cellular processes such as tissue regeneration, repair, and disease progression.
Strategy of inducible fate mapping (Peterson, J.C., et al. 2021)
In inducible fate mapping, an inducible promoter is used to control the expression of a site-specific recombinase such as Cre or Flp. This allows for the activation of gene expression in specific subsets of cells in response to an external stimulus or manipulation. For example, the promoter could be activated by the administration of a drug, exposure to light, or changes in temperature. When the promoter is activated, the recombinase will excise a DNA fragment that is flanked by specific recognition sequences, resulting in the expression of a marker gene such as GFP.
Advantages of Inducible Fata Mapping
Compared with traditional fate mapping technologies, inducible fate mapping has several advantages, followings are some main advantages of this technology.
| Temporal control of gene expression | With this technology, researchers can precisely control the timing of gene expression in specific cells. Particularly for studying dynamic biological processes, such as tissue regeneration. |
| Specificity of gene expression | Inducible fate mapping allows for the activation of specific gene expression, as well as studying the fate and lineage of specific cells. And this technology can also be applied in complex tissues and organs, such as the nervous system or immune system, where individual cell types may be difficult to isolate and study. |
| Flexibility in experimental design | Inducible fate mapping can be designed to respond to a wide variety of stimuli, such as exposure to specific drugs or changes in temperature or light. This provides flexibility in experimental design and allows for the study of a wide range of biological processes. |
| Reduced toxicity and genetic mosaicism | This technology can reduce the potential toxicity or genetic mosaicism associated with constitutive gene expression. By activating gene expression only in specific cells or specific time, the off-target effects or unwanted gene expression can be reduced. |
| Wide range of applications | Inducible fate mapping has broad applications in disease research and therapy, allowing researchers to study disease processes and develop new therapeutic strategies. |
Our Service
Base on the T-SSR-mediated inducible fate mapping technology, Creative Biogene offers services included but not limited as follows:
- Cell lineage tracing service
Creative Biogene offers cell lineage tracing service for track the lineage of specific cell populations over time, providing insights into developmental processes and tissue homeostasis. - Disease modeling service
We provide T-SSR-mediated disease modeling service for study disease progression and identify potential therapeutic targets. - Regenerative medicine development service
Based on T-SSR-mediated inducible fate mapping technology, we provide tissue regeneration and repairment development service, which can tell the fate of specific cell populations during the regenerative process. - Gene therapy assessment service
Creative Biogene offers gene therapy assessment service based on our T-SSR-mediated inducible fate mapping technology, in which the specific cell population can be labeled.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
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Peterson, J.C., et al. The Role of Cell Tracing and Fate Mapping Experiments in Cardiac Outflow Tract Development, New Opportunities through Emerging Technologies. J. Cardiovasc. Dev. Dis. 2021, 8, 47.