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Customized Modeling Service
Customized modeling services use the site-specific recombinase system to precisely and efficiently make the desired genetic modifications. This is done by introducing site-specific recombinase into the genome of the organism, which then recognizes and recombines with specific sites in the genome. By using different optimized site-specific recombinase system, Creative Biogene can perform specific genetic modifications to the genome as our global customers require.
Development of Modeling via T-SSR System
Site-specific recombinases are enzymes that catalyze the exchange of DNA sequences between two defined sites in a highly specific and efficient manner. These enzymes recognize and bind to short DNA sequences, called recognition specific sites, and bring them together, allowing for a precise recombination event to occur. Cre/loxP is one of the most widely used technologies among them.
The original Cre-loxP technology allows for conditional gene knockout, meaning that researchers can delete specific genes in a specific tissue or at a specific time. This is achieved by introducing loxP sites flanking the gene of interest and expressing Cre recombinase in the desired tissue or at the desired time. When Cre recombinase recombines with the loxP sites, the gene of interest is deleted.
Mechanism of Cre-loxP system (Kim, Hyeonhui et al., 2018)
In recent years, modified Cre-loxP technology has been developed that allows for even more precise and versatile genome engineering. This includes the use of different Cre recombinases with different properties, and the use of different loxP site combinations to perform specific recombination events.
What’s more, many T-SSR-based technologies have been developed for solving multiple problems that occur in genetic engineering, such as specificity, precision and efficiency.
Technologies for Customized Modeling
Among technologies that are widely used in conditional genetic engineering, T-SSR system has some advantages over the others in terms of precision, efficiency, and flexibility. It allows for the spatial and temporal control of gene deletion/insertion with high efficiency and precision, making it a powerful tool for studying gene function in vivo. The system is also highly flexible, allowing for the generation of various mouse models for different research purposes. Additionally, the T-SSR system is well-established, with a large body of literature and a wide range of available tools and resources for its use. Followings are comparison of popular technologies.
| Technology | Introduction | Advantages |
|---|---|---|
| Site-specific Recombinase | Site-specific recombinase technology, such as Cre/loxP system, which can achieve the deletion of specific genes in a tissue-specific or temporal-specific manner with high efficiency and accuracy. | Precise and efficient gene deletion with spatial and temporal control |
| CRISPR/Cas9 | CRISPR/Cas9 can introduce precise double-stranded breaks in specific genomic regions and induce gene knockouts or knock-ins | Highly specific and customizable genome editing |
| TALENs | Zinc finger nucleases (Zinc Finger Nucleases) and transcription activator-like effector nucleases (TALENs), which can also introduce site-specific double-stranded breaks and induce gene knockout or knock-in. | Similar to CRISPR/Cas9, but can recognize different DNA sequences |
| Zinc Finger Nucleases | Similar to TALENs, but less efficient and more complex to design and optimize |
Our Service
Our well-developed platform CreEditTM has years of experience about genetic engineering in various of species included but not limited as follows.
Based on these optimized T-SSR technologies, Creative Biogene provides customized modeling services including conditional KO, inducible KI, reporter model, and others.
Our site-specific recombinase technologies can be used to generate conditional knockout, allowing for the tissue-specific and temporal control of gene expression.
Our site-specific recombinase technologies can also be used to control gene expression by regulating the presence or absence of specific DNA sequences.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Kim, Hyeonhui et al. "Mouse Cre-LoxP system: general principles to determine tissue-specific roles of target genes." Laboratory animal research vol. 34,4 (2018): 147-159.