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Customized Microbial Models
Microbes are powerful tools that have a wide range of applications in biological research. The site-specific recombinase allows researchers insert, delete, or modify specific DNA sequences within the genome of a microbe, as well as the precise control of gene expression and the creation of new genetic constructs. Creative Biogene offers customized microbial model development service for assisting out global customers making better breakthrough in biology research.
Introduction into Microbial Models Generated by SSR
Site-specific recombinases are widely used in microbes for genome engineering and manipulation. Microbes are ideal candidates for this technology due to their small and relatively simple genomes, ease of genetic manipulation, and the ability to grow and manipulate them in large quantities.
One of the most commonly used site-specific recombinases in microbes is Cre recombinase, which can be used to catalyze the site-specific recombination of loxP sites in bacterial and yeast genomes. This technology allows for the precise manipulation of bacterial and yeast genomes, enabling the creation of strains with specific genetic modifications or characteristics.
Strategy of marker
removal genomic engineering in yeast (Zhang, Ning et al. 2019)
Advantages of SSR in Microbes
- Site-specificity: Site-specific recombinases can target specific DNA sequences, such as loxP sites, ensuring that genetic modifications are made only at the desired location in the genome. This enables precise and controlled genetic engineering, minimizing the risk of unintended effects on the host cell.
- Reversibility: Site-specific recombinases are reversible, meaning that genetic modifications can be undone by simply expressing the recombinase again. This allows for the creation of conditional genetic modifications, where genes can be turned on or off depending on the expression of the recombinase.
- Efficiency: Site-specific recombinases are highly efficient, enabling rapid and reliable genetic modifications with minimal off-target effects. This allows for the rapid generation of large numbers of genetically modified microbial strains for use in research or industrial applications.
- Scalability: Site-specific recombinase technology is scalable, allowing for the generation of large numbers of genetically modified microbial strains for use in high-throughput screening or industrial production. This enables the rapid development of new biotechnological applications and the efficient optimization of existing processes.
- Compatibility: Site-specific recombinase technology is compatible with a wide range of microbial species, enabling the generation of genetically modified strains across a broad range of applications. This makes it a versatile tool for microbial biotechnology.
Our Service
Creative Biogene offers customized microbial model development service based one our well-developed site-specific recombinase technologies and our years of genetic engineering experience. We provide services in microbes included but not limited as follows:
E. coli
Yeast
Slime Mold
Cyanobacteria
B. subtilis
Creative Biogene offers a cutting-edge solution for targeted gene editing in bacteria and other microbes. With our expertise in site-specific recombinase technology, we can generate precise and controlled gene knockouts that allow you to study the effects of specific genes on microbial physiology, metabolism, and bioproduction. Please view the following pages to check out the details of our service.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Zhang, Ning et al. Selectable marker recycling in the nonconventional yeast Xanthophyllomyces dendrorhous by transient expression of Cre on a genetically unstable vector. Applied microbiology and biotechnology vol. 103,2 (2019): 963-971.