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Customized Plant Models
The site-specific recombinase systems commonly used in plant modeling. The Cre/loxP system is a powerful genetic tool that has been widely used to manipulate DNA sequences in a site-specific manner in plants, including model plants, cash crops and others. Creative Biogene offers customized plant model generation service based on out advanced and well-developed SSR technology.
Introduction into SSR in Plant Modeling
In plant modeling, the SSR system has been used to generate conditional gene knockouts and gene editing lines. By introducing specific sites into the genome of the plant, the recombinase can be used to excise or invert specific DNA segments, leading to the loss or gain of gene function. Cre/loxP system is one of the most popular SSR system that has been applied in plant modeling, especially generating conditional gene knockouts and gene editing lines.
By using tissue-specific promoters to drive tissue-specific recombinase expression, researchers can excise a floxed gene specifically in a desired tissue, allowing them to study the function of that gene in that tissue. This method can help overcome some of the limitations of traditional knockout studies, which often result in lethal phenotypes that make it difficult to study gene function.
General strategy for optogenetic systems
in plants (Omelina, Evgeniya S et al., 2022)
Applications of SSR in Plants
- Gene knockouts: The SSR system has been used to generate gene knockouts in plants. By placing specific sites around a target gene, researchers can use recombinase to excise the gene, resulting in a loss of gene function. This approach has been used to study the functions of many genes in plants, including those involved in plant development, stress responses, and metabolism.
- Gene editing: The SSR system has also been used for gene editing in plants. By placing a specific site around a target gene, researchers can use recombinase to excise the gene and replace it with a modified version. This approach has been used to introduce new traits into plants, such as disease resistance, improved nutrient uptake, and increased yield.
- Conditional gene expression: The SSR system has been used to regulate gene expression in a temporal and spatial manner in plants. By placing a specific site upstream of a target gene, researchers can use Cre recombinase to excise the specific site and activate gene expression. This approach has been used to study the roles of specific genes in plant development and to engineer plants with improved stress tolerance.
- Cell type-specific gene expression: The SSR system has also been used to drive gene expression in specific cell types in plants. By using cell type-specific promoters to drive recombinase expression and specific sites to control gene activation, researchers can selectively express genes in specific cell types. This approach has been used to study the functions of genes in specific tissues and to engineer plants with improved traits, such as increased photosynthetic efficiency.
Our Service
Based on our optimized site-specific recombinase system, we offer plant modeling services in plants included but not limited as follows.
With the optimized SSR technologies we've developed for solving all kinds of problem we might face in plant genetic engineering, Creative Biogene offer plant modeling services as follows:
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Omelina, Evgeniya S et al. "Optogenetic and Chemical Induction Systems for Regulation of Transgene Expression in Plants: Use in Basic and Applied Research." International journal of molecular sciences vol. 23,3 1737. 3 Feb. 2022.