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STOCK Gt(ROSA)26Sortm1(CAG-EYFP*, -mEYFP*, -tdTomato*, -mTFP1*)Ben/Gt(ROSA)26Sortm2(CAG-EYFP*, -mCherry*, -EGFP*, -mCerulean*)Ben/J
Cat. No.: CEMM-07250853
Common Name: iMb2-Control-Mosaic::iChr2-Control-Mosaic
iMb2-Control-Mosaic::iChr2-Control-Mosaic compound heterozygous mice allow several possible Cre recombination outcomes in any single cell. Each outcome results in expression of both a membrane-bound fluorescent protein and a chromatin/nuclear-localized fluorescent protein, and each is mutually exclusive and permanent. These mice are also designed to permit further introduction of genes of interest for inducible, fluorescent and functional genetic mosaic analysis (ifgMosaic or Dual ifgMosaic).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm2(CAG-EYFP*,-mCherry*,-EGFP*,-mCerulean*)Ben
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| Allele Name |
targeted mutation 2
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Epitope tag
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral; YFP, Yellow Fluorescent Protein, jellyfish; CFP, Cyan Fluorescent Protein, jellyfish; HA tag
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| MGI Accession ID | show more close |
| Site of Expression |
Fluorescent proteins will be expressed in in tissues expressing Cre recombinase driven by a selected promoter.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
This allele has three chromatin/nuclear-targeted fluorescent protein sequences uniquely flanked with pairs of incompatible lox sites (in the same direction), all inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. The targeting vector is designed with (from 5' to 3') two copies of chicken beta-globin HS4 insulator element (2xINS; inCrease downstream gene expression and minimize regulatory interference), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxN site, a lox2272 site, a loxP site, the N-PhiMut gene (non-cytotoxic/non-fluorescent Phialidium yellow fluorescent protein variant PhiYFP with nuclear localization signal), SV40 polyA signal sequence, a second loxN site, a full consensus KOZAK sequence, the 6xHis-H2B-mCherry gene (monomeric DsRed fluorescent protein variant mCherry with N-terminal 6xHis affinity tag and 125 amino acid human histone 2B chromatin/nucleus tag), in-frame Thosea asigna virus 2A cleavage site (T2A; mediates ribosomal skipping), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance mRNA transcript stability), SV40 polyA signal sequence, a second lox2272 site, a full consensus KOZAK sequence, the H2B-EGFP gene (enhanced green fluorescent protein with 127 amino acid human histone 2B chromatin/nucleus tag and KOZAK sequence plus C-terminal paramyxovirus V5 tag sequence), in-frame T2A sequence, WPRE sequence, SV40 polyA signal sequence, a second loxP site, a full consensus KOZAK sequence, the HA-H2B-mCerulean gene (monomeric enhanced cyan fluorescent protein variant mCerulean with N-terminal hemagglutinin (HA) tag, 125 amino acid human histone 2B chromatin/nucleus tag and KOZAK sequence), in-frame T2A sequence, WPRE sequence, SV40 polyA signal sequence, an FRT site flanked neomycin resistance gene cassette, and a second 2xINS sequence.
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For Research Use Only.
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