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Site-specific Recombinase Technology
The site-specific recombinase system is a genetic tool used in molecular biology to precisely modify the DNA sequence of a cell. This system uses enzymes called recombinases, which can recognize specific DNA sequences and carry out recombination events, such as insertion, deletion, or exchange of DNA fragments.
What Is Site-specific Recombinase System?
Site-specific recombinases are DNA modifying enzymes that identify specific target sites and bind, cleave, strand exchange, and rejoin DNA between the sites. They are commonly used as molecular tools in biotechnology, genetic engineering, and synthetic biology. It was first found in bacteriophage lambda, which utilize a protein called lambda-integrase (Int) to integrate and excise the genome into and out of the bacterial chromosome. After that, the widely-used Cre and Flp were first described in research. They don't require auxiliary proteins to organize the synaptic complexes that initiate the recombination response, which allows them to work in heterologous host easily. Due to this fact, they are widely used as tools for a large quantity of applications, as well as for generating many model organisms.
Schematic representation of SSR recombination (Meinke,
Gretchen et al. 2016)
What Can Site-specific Recombinase System Do?
Based on the direction of target sites, the recombination events generally have four outcomes: excision, inversion, translocation and cassette exchange.
Excision: When target sites are in the same direction on the same DNA strand, the recombination results in excision of the DNA region between the sites.
Inversion: When the target sites are in the opposite direction on the same DNA strand, the recombination results in inversion of the DNA region between the sites.
Translocation: The translocation happens when the two target sites are on the separate DNA molecules.
Cassette exchange: When target gene is flanked by two different specific sites, in the presence of a DNA molecule flanked by the same two different sites, the cassette exchange happens.
In genetic engineering and biotechnology, tyrosine site-specific recombinases are often used in combination with other tools, such as inducible promoters or conditional alleles, to achieve temporal or spatial control over the recombination event. This allows researchers to precisely control when and where the recombination occurs, enabling them to study the function of specific genes or to generate transgenic animals.
Optimized Site-specific Recombinase-based Strategies
For assisting our global customers making greater breakthrough in their research, Creative Biogene has developed and optimized site-specific recombinase system with strategies as followings. Please check up the pages for the detailed information.
Why Choose Us
One-stop Service
High-efficiency
Professional Team
High-quality
Creative Biogene has years of experience in site-specific recombinase-based applications. We have established the advanced CreEditTM platform, which aims to support our global customers with high-quality, cost-effective and high-precision one-stop services. Our services are not limited in what we mentioned above, please feel free to contact us and get started with our first-class services.
Reference
- Meinke, Gretchen et al. "Cre Recombinase and Other Tyrosine Recombinases." Chemical reviews vol. 116,20 (2016): 12785-12820.