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STOCK Stag1tm1c(EUCOMM)Hmgu/J
Cat. No.: CEMM-07250834
Common Name: Stag1flox
The "conditional ready" (floxed) Stag1tm1c(EUCOMM)Hmgu allele (Stag1flox) has loxP sites flanking exon 7. Exposure to Cre recombinase creates the knock-out allele. These mice may be useful in studying the cohesin complex and STAG1 loss-of-function mutations in diseases such as neurodevelopmental disorders.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Stag1tm1c(EUCOMM)Hmgu
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| Allele Name |
targeted mutation 1c
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| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
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| Gene Symbol |
Stag1
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| Gene Name |
stromal antigen 1
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| Chromosome |
9
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| MGI Accession ID | show more close |
| Strain of Origin |
C57BL/6N-Atm1Brd
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| Molecular Note |
The L1L2_Bact_P cassette was inserted upstream of the critical exon (exon 7) of the stromal antigen 1 locus (Stag1) - with insertion at bp position Chr9:100,785,969 [Build GRCm38]. The L1L2_Bact_P cassette was composed of an FRT site, a mouse En2 intron/splice-acceptor/exon (En2 SA), an IRES sequence, the beta-galactosidase gene (lacZ) with SV40 polyadenylation signal (SV40pA), a loxP site, the human beta actin promoter-driven neomycin-resistance gene with SV40 polyadenylation signal (hbactP-neo-pA), a second FRT site and a second loxP site. A third loxP site was inserted downstream of exon 7 at bp position Chr9:100,786,980 [Build GRCm38]; therefore flanking exon7 with loxP sites. Flp-mediated recombination removed the FRT flanked neo cassette leaving exon 7 floxed.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Mice homozygous for the floxed allele (Stag1flox/flox) are viable and fertile, with no reported gross phenotypic abnormalities or breeding problems. When maintaining a live colony, homozygous mice may be bred together.
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| Breeding Strategy |
Heterozygote x Heterozygote
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For Research Use Only.
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