C57BL/6-Tg(Slc6a3-icre/ERT2)2Gloss/J

Cat. No.: CEMM-07250312

Common Name: DAT-iCreER

These DAT-iCreER transgenic mice express a tamoxifen-inducible Cre recombinase under the control of the mouse Slc6a3 promoter region and may be useful for generating conditional mutations for studying gain or loss of function and/or fate mapping of dopaminergic neurons in the substantia nigra and locus coeruleus, as well for studying drug addiction, Parkinson's disease and Attention Deficit-Hyperactivity Disorder (ADHD).

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Slc6a3-icre/ERT2)2Gloss show more close
Allele Name	transgene insertion 2 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Tg(Slc6a3-icre/ERT2)2Gloss show more close
Gene Name	transgene insertion 2 show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1; ESR1, estrogen receptor 1, human show more close
MGI Accession ID	5049906 show more close
Site of Expression	Tamoxifen administration induces Cre recombination in dopaminergic cells of the substantia nigra and locus coeruleus. show more close
Strain of Origin	C57BL/6NTac show more close
Molecular Note	The mouse bacterial artificial chromosome (BAC) RP23-408F13 containing Slc6a3, solute carrier family 6 (neurotransmitter transporter, dopamine), member 3, gene, was modified by insertion of sequence encoding iCreER at the Slc6a3 translation initiation ATG, and addition of a Kozak sequence upstream of the ATG. This BAC also contains the following genes: Clptm1l (CLPTM1-like), Tert (telomerase reverse transcriptase), and Slc6a18 (solute carrier family 6 (neurotransmitter transporter), member 18). The modified BAC was used to generate the DAT-iCreER transgene, which was microinjected into C57BL/6NTac fertilized eggs. Founder line 2 was subsequently established. show more close

HUSBANDRY
Suggested Controls	C57BL/6NJ show more close
Breeding Considerations	When maintaining a live colony, these mice can be bred as homozygotes. show more close

For Research Use Only.