STOCK Tg(CAG-Bgeo/GFP)21Lbe/J

Tg(CAG-Bgeo/GFP)21Lbe

transgene insertion 21

Conditional ready (e.g. floxed); Reporter

Tg(CAG-Bgeo/GFP)21Lbe

transgene insertion 21

5

GFP, Green Fluorescent Protein; Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli; lacZ, beta-galactosidase, E. coli

3046177

LacZ is widely expressed throughout embryonic and adult tissues with notable exceptions being liver and lung; When crossed with a Cre recombinase-expressing strain, LacZ expression is replaced with Enhanced Green Fluorescent Protein expression

(129X1/SvJ x 129S1/Sv)F1-Kitl+

This transgene contains a CMV enhancer/chicken beta actin promoter, a segment consisting of floxed lacZ and neomycin resistance genes/SV40 polyadenylation signal, and an enhanced GFP reporter gene followed by a rabbit beta-globin polyadenylation sequence. Expression of lacZ in transgenic mice is widespread, with notable exceptions being liver and lung tissue. This expression is observed throughout all embryonic and adult stages. Upon exposure to Cre recombinase to excise the lacZ-polyA cassette, EGFP is expressed in Cre dependant manner. This transgene was inserted in the G1 band of Chromosome 5 at approximately 136.35 MB (build 37).

Noncarrier

When maintaining a live colony, hemizygous mice are bred with wildtype (non-carrier) mice. Although homozygotes are viable, attempts to breed homozygous mice proved unsuccessful.

Noncarrier x Hemizygote

Mice from this stock are albino