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STOCK Slc6a3tm1(cre)Xz/J

Cat. No.: CEMM-07250424

Common Name: DAT-cre
The DAT-cre "knock-in" allele has a nuclear-localized Cre recombinase inserted just upstream of the first coding ATG of the dopamine transporter gene (Slc6a3; DAT). This was designed to both abolish DAT gene function and place Cre recombinase expression under the control of the DAT promoter/enhancer elements.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Slc6a3tm1(cre)Xz
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Allele Name
targeted mutation 1
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Allele Attributes
Recombinase-expressing
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Gene Symbol
Slc6a3
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Gene Name
solute carrier family 6 (neurotransmitter transporter
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Chromosome
13
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Expressed Genes
Cre, Cre recombinase, bacteriophage P1
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MGI Accession ID
3691571
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Site of Expression
Dopaminergic neurons
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Strain of Origin
129S1/Sv-Oca2+ Tyr+ Kitl+
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Molecular Note
A targeting vector was constructed by inserting a cassette with the Cre recombinase coding sequence, a nuclear translocation signal, and a PGK-neo gene flanked by FRT sites, into the 5'-UTR of the Slc6a3 gene. After homologous recombination in ES cells, mice with Cre recombinase expressed from the Slc6a3 gene were produced. Knockin mice were crossed with transgenic Flp deleter mice to remove the neo marker. A line was established where the neo was removed and the Flp transgene was absent. Cre recombinase expression is detected in the ventral tegmental area (VAT) and substantia nigra zona compacta (SNc).
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HUSBANDRY
Suggested Controls
Wild-type from the colony
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Breeding Considerations
Heterozygotes are viable and fertile. Homozygotes are not healthy.
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Breeding Strategy
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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