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B6.FVB-Bdnfem1(cre)Zak/J
Cat. No.: CEMM-07250781
Common Name: BDNF-2A-Cre knock-in
BDNF-2A-Cre knock-in mice are designed to have Cre recombinase expression directed to brain derived neurotrophic factor-expressing cells. Cre activity recapitulates that of the endogenous Bdnf gene in the brain. These mice may be used to generate conditional mutations for studying this neuropeptide/neurotrophin in aspects of neuronal function, including growth/differentiation of neurons and synapses, neurogenesis, synaptic transmission, learning, long-term memory, heat responses and human disease.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Bdnfem1(cre)Zak
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| Allele Name |
endonuclease-mediated mutation 1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Bdnf
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| Gene Name |
brain derived neurotrophic factor
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| Chromosome |
2
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre recombinase activity largely recapitulates the endogenous Bdnf gene in brain regions such as ventromedial, paraventricular and preoptic hypothalamus, as well as cortex, and CA1 and dentate gyrus of the hippocampus.
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| Strain of Origin |
FVB/NJ
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| Molecular Note |
The bicistronic BDNF-2A-Cre knock-in allele was Created using homologous recombination (aided by targeted CRISPR/Cas9 endonuclease activity) to insert a viral 2A oligopeptide sequence (T2A; mediates ribosomal skipping) and a Cre recombinase gene (Cre) just upstream of the endogenous STOP codon of the brain derived neurotrophic factor locus (Bdnf). The targeting vector, Cas9 endonuclease and single guide RNA were mixed and injected into the pronuclei of FVB/NJ fertilized zygotes. Embryos were transferred to pseudopregnant females, and correctly targeted pups were bred to C57BL/6J inbred mice for germline transmission.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities - and homozygous mice may be bred together.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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