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STOCK En1tm7(cre/ESR1)Alj/J
Cat. No.: CEMM-07250083
En1-CreERT1 mutant mice harbor the Cre-ERT1 fusion gene under control of the endogenous engrailed 1 locus. When En1-CreERT1 mice are bred with mice containing a loxP-flanked sequence of interest, tamoxifen-inducible, Cre-mediated recombination will result in deletion of the flanked sequences in En1-expressing tissues (including the mesencephalon and rhombomere 1, as well as the developing cerebellum, dermis, and limbs).
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
En1tm7(cre/ESR1)Alj
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| Allele Name |
targeted mutation 7
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| Allele Attributes |
Recombinase-expressing; Inducible
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| Gene Symbol |
En1
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| Gene Name |
engrailed 1
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| Chromosome |
1
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| Expressed Genes |
cre/Esr1, Cre recombinase and estrogen receptor 1 fusion gene
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| MGI Accession ID | show more close |
| Site of Expression |
Mesencephalon and rhombomere 1, as well as the developing cerebellum, dermis, and limbs (after induction)
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| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
A tamoxifen dependent form of Cre recombinase (CreER) and a loxP-flanked neo in reverse orientation were inserted into the locus. The neo was removed in vivo via Cre-mediated recombination.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
When maintaining a live colony, heterozygous mice may be bred to wildtype siblings. The donating investigator reports that homozygotes die at birth. Of note, to ensure the mutant phenotype of these En1-mutant mice, avoid crossing these mice onto the C57BL/6 genetic background (as the phenotype of En1-null mice is rescued on the C57BL/6 genetic background).
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For Research Use Only.
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