B6;129-Igs1^{tm5(CAG-Bgeo, -Mito-GFP)Nat}/J

Igs1^{tm5(CAG-Bgeo,-Mito-GFP)Nat}

targeted mutation 5

Conditional ready (e.g. floxed); Reporter; No functional change

Igs1

intergenic site 1

11

Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli

5319536

Upon Cre- mediated recombination in various cell types, moderate to strong native GFP fluorescence is detected specifically in mitochondria both proximal and distal to the cell body. GFP expression is especially robust in endothelial cells residing in the brain and retina.

129

The following elements were inserted, in order, 2 kb 5' of the Ubb gene by targeted recombination in 129-derived ES cells: CMV enhancer/beta-actin promoter (CAG), loxP site, beta-geo, 3 transcription sites, loxP site, mitochondria targeted GFP fused to a C-terminal V5 epitope, and a bovine growth hormone 3' UTR. Mitochondrial targeting was achieved by using the N-terminal 25 amino acid targeting signal of mouse Cox8a. The V5 epitope encodes amino acid residues 95-108 (GKPIPNPLLGLDST) of RNA polymerase alpha subunit of simian virus 5.

Heterozygotes are viable and fertile, but homozygotes are unhealthy.