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STOCK Slc25a5tm1.1Nte/J

Cat. No.: CEMM-07250734

Common Name: Ant22f (floxed exons 2-3)
The Ant22f (floxed exons 2-3) allele allows Cre-conditional knockout of the adenine nucleotide translocase 2 gene (Slc25a5 or Ant2) on the X chromosome. Removal of the floxed sequence creates a null allele. These mice are useful for spatiotemporal deletions of Ant2 in studying energy metabolism, mitochondrial dysfunction and oxidative stress, as well as cancer or developmental disorders.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Slc25a5tm1.1Nte
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Allele Name
targeted mutation 1.1
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Allele Attributes
Conditional ready (e.g. floxed); No functional change
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Gene Symbol
Slc25a5
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Gene Name
solute carrier family 25 (mitochondrial carrier
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Chromosome
X
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MGI Accession ID
5790470
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Strain of Origin
129S4/SvJae
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Molecular Note
A targeting vector was designed to insert a loxP site upstream of exon 2, and an FRT splice donor/acceptor-IRES-beta-gal/neo-FRT loxP downstream of exon 3. ES cells were transiently transfected with a FLP-expressing plasmid to remove the SA-IRES-betageo cassette leaving exons 2-3 floxed.
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HUSBANDRY
Suggested Controls
B6129SF2/J C57BL/6J
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Breeding Considerations
The Ant22f allele is on the X chromosome. Homozygous females (Ant22f/2f) and hemizygous males (Ant22f/Y) are viable and fertile with no reported abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. Alternatively, homozygous females may be bred with hemizygous males.
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For Research Use Only.
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