B6;129S-Esr2^{tm1.1(cre)Hze}/J

Esr2^{tm1.1(cre)Hze}

targeted mutation 1.1

Recombinase-expressing

Esr2

estrogen receptor 2 (beta)

12

Cre, Cre recombinase, bacteriophage P1

5904044

When these mice are bred with mice containing lox-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in Esr2-expressing cells in the offspring.

(129S6/SvEvTac x C57BL/6NCrl)F1

The targeting vector contained, from 5' to 3', a partial Esr2 sequence spanning exon 10 (including the endogenous stop codon), an internal ribosome entry site 2 (IRES2) sequence (allows translation initiation in the middle of an mRNA sequence), a Cre recombinase gene, a bovine growth hormone polyA sequence, an AttB site, a PGK promoter-Neomycin resistance gene-PGK polyA cassette, an frt5 site, an mRNA splice acceptor sequence, the 3' portion of the hygromycin gene (Hygro2) with SV40 polyA signal, and an AttP site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence (PGK-Neo-polyA::FRT5::RNA splice acceptor::3'hygro-polyA) and replaced it with the recombined AttB/AttP site (AttL). The resulting Esr2-IRES2-Cre-D mice were bred with C57BL/6J wildtype mice. Cre recombinase expression/activity largely recapitulates the endogenous Esr2 gene expression in the brain - observed mainly in medial amygdalar nucleus, bed nuclei of the stria terminalis, paraventricular hypothalamic nucleus, cortical layer 6 and scattered neurons elsewhere in the brain.

C57BL/6J

When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice. it has not been attempted to make this strain homozygous.

Wild-type x Heterozygote; Heterozygote x Wild-type