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STOCK Notch1tm5(cre/ERT2)Rko/J

Cat. No.: CEMM-07250633

Common Name: N1IP::CreERT2
The tamoxifen-inducible Notch1 activation-dependent reporter knock-in allele, N1IP::CreERT2, has the endogenous Notch1 intracellular domain (NICD1) replaced with a 6xMyc-tagged, tamoxifen-inducible Cre recombinase (6mtCreERT2). Similar to the N1IP::CreLO allele, this N1IP::CreERT2 allele is predominantly expressed in cells in which moderate-to-high levels of sustained Notch activity or repeated activation cycles are known to occur (e.g., endothelium), with the added benefit that Cre recombinase activity is tamoxifen-inducible.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Notch1tm5(cre/ERT2)Rko
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Allele Name
targeted mutation 5
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Allele Attributes
Recombinase-expressing; Inducible
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Gene Symbol
Notch1
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Gene Name
notch 1
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Chromosome
2
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Expressed Genes
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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MGI Accession ID
5009237
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Site of Expression
The cre/ERT2 fusion protein is expressed on the cell membrane of cells in which Notch1 activity occurs, such as the endothelium.
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Strain of Origin
C57BL/6NTac-Tg(HBB-lacZ)ALey/Ley
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Molecular Note
The targeting construct was designed to replace exons 29-34 (encoding the intracellular domain) with a CreERT2 sequence and nuclear localization signal, followed immediately downstream by a C-terminal 6xMyc epitope tag, 2xSV40 polyadenylation sequence, and FRT flanked Pgk1/EM7 promoter-driven neomycin selection cassette. The CreER fusion gene (Cre-ERT2) is Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain.
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HUSBANDRY
Suggested Controls
Wild-type from the colony
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Breeding Considerations
When maintaining a live colony, heterozygous mice are bred to wildtype mice from the colony. Homozygotes die in utero.
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For Research Use Only.
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