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B6.129S6-Eef1a1tm1Klan/J
Cat. No.: CEMM-07251069
Common Name: Eef1a1LSL.NS3/4.TRAP.iPKR; iPKR; Eef1a1-LSL-A2GA5
iPKR knock-in/knock-out mice were developed for cell type-specific drug-inducible protein synthesis inhibition (ciPSI). They are a spatiotemporally precise chemogenetic tool for rapidly and reversibly blocking cell-autonomous protein synthesis via phosphorylation of eIF2α (EIF2A). Expression of Eef1a1 is knocked out by the targeted insertion and homozygotes are not viable.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Eef1a1tm1Klan
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Reporter; Inserted expressed sequence
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| Gene Symbol |
Eef1a1
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| Gene Name |
eukaryotic translation elongation factor 1 alpha 1
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| Chromosome |
9
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| MGI Accession ID | show more close |
| Strain of Origin |
129S6/SvEvTac
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| Molecular Note |
The targeting cassette introduced into intron 1 a floxed stop cassette, a recombinant nonstructural protein recognition site NS3/4A (allows for chemical inhibition with asunaprevir), self-cleaving 2A proteinase sequence, ribosomal localized EGFP reporter gene (EGFP/L10), self-cleaving 2A proteinase sequence, and an inducible kinase domain from an RNA-activated protein kinase (iPKR).
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Heterozygotes are viable and fertile. Homozygotes are not viable.
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| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
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For Research Use Only.
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