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STOCK Igs7tm86(tetO-CiVS/pHluorin)Hze/VpiJ
Cat. No.: CEMM-07250991
Common Name: Ai86(TITL-ArcLight)
Ai86(TITL-ArcLight) mice are a Cre/Tet-dependent, voltage-sensitive, fluorescent line, created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). After removal of the floxed-STOP cassette by Cre recombinase, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of the voltage-sensitive fluorescent protein ArcLight. ArcLight exhibits voltage-dependent FRET (Förster resonance energy transfer pairs) upon membrane depolarization, and enables optical recordings at high spatial resolution with intrinsic assignment of recorded signals to targeted cell populations.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm86(tetO-CiVS/pHluorin)Hze
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| Allele Name |
targeted mutation 86
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector contains (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a modified Tet response element (TRE or tetO), a loxP-STOP-loxP cassette, the voltage-sensitive fluorescent protein Butterfly 1.2 coding sequence (referred to as ArcLight - details below), a WPRE sequence (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site inserted into the Igs7 locus (T1 TIGRE - an intergenic region on chromosome 9 [between AB124611 and Carm1]) using recombinase-mediated cassette exchange (RMCE).The voltage-sensitive fluorescent protein ArcLight is a fusion of a CiVS [Ciona intestinalis voltage sensor (S1-S4 transmembrane segment, including the voltage-sensing domain in S4)] and the fluorescent protein super ecliptic pHluorin (an eGFP variant differing from eGFP at 9 positions) with an A227D mutation, and exhibits voltage-dependent Forster resonance energy transfer (FRET) upon membrane depolarization. In this strain, the fluorophore is inserted at position A242 within the CiVS.
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For Research Use Only.
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