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B6.Cg-Gt(ROSA)26Sortm3.2(CAG-EGFP, -CHRM3*/mCherry/Htr2a)Pjen/J
Cat. No.: CEMM-07250622
Common Name: RC::FL-hM3Dq
This strain is currently unavailable due to replenishing of cryopreserved stocks. Interested customers can register interest.RC::FL-hM3Dq is a dual recombinase-responsive Gq-coupled DREADD allele with a frt-flanked STOP and a cre-dependent FLEx switch containing an eGFP sequence and an inverted hM3Dq/mCherry/2ACT88 fusion protein. Flp-recombinase results in widespread, robust eGFP fluorescence, and further exposure to Cre recombinase may replace eGFP expression with somatodendritic hM3Dq/mCherry expression. The DREADD hM3Dq induces the canonical Gq pathway (depolarization/activation) specifically following administration of its pharmacologically inert ligand (CNO). The RC::FL-hM3Dq allele and its derivatives permit chemogenetic/pharmacogenetic activation of an intersectional subpopulation defined by overlap of Flp and Cre recombinase expression.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3.2(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen
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| Allele Name |
targeted mutation 3.2
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; RFP, Red Fluorescent Protein, coral; Htr2a, 5-hydroxytryptamine receptor 2A, rat
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| MGI Accession ID | show more close |
| Site of Expression |
EGFP fluorescence is widespread after removal of the STOP sequence by FLP recombination. mCherry fluorescence is expressed in Cre expressing tissues after Cre recombination.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The allele has a CAG hybrid promoter,a rox-flanked His3-SV40 transcriptional STOP cassette, an FRT flanked STOP cassette and a variant lox-flanked region containing an eGFP cassette, an inverted hM3Dq/mCherry fusion protein, WPRE, polyA signal and an attB/attP-flanked PGK-FRT Neo-polyA cassette all inserted into the Gt(ROSA)26Sor locus. The inverted hM3Dq-mCherry-2ACT88 fusion protein cDNA sequence contains (hM3Dq DREADD) a red fluorescent protein mCherry and the final 264 coding nucleotides of the rat Htr2A gene (encoding a C-terminal epitope that mediates localization to the cell body/soma and dendrites). PhiC31-mediated recombination removed the AttB/AttP-flanked neo sequence. Dre-mediated recombination removed the rox-flanked STOP cassette. Flp-mediated recombination removed FRT flanked STOP cassette.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together. Note, although the donating investigator reports that mice were backcrossed onto C57BL/6J for five generations prior to sending to CD BioScience Repository, they did not select pffspring for coat color - and this colony may exhibit agouti or black coat color.
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For Research Use Only.
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