Online Inquiry
B6.129(FVB)-Gt(ROSA)26Sortm1(CAG-birA, -EGFP)Joez/J
Cat. No.: CEMM-07250803
Common Name: cBirA
cBirA mice are designed to express BirA (biotin ligase) and EGFP following exposure to Cre recombinase. This is a tool for the isolation and purification of proteins and protein complexes from tissues.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-birA,-EGFP)Joez
show more
close
|
| Allele Name |
targeted mutation 1
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; No functional change
show more
close
|
| Gene Symbol |
Gt(ROSA)26Sor
show more
close
|
| Gene Name |
gene trap ROSA 26
show more
close
|
| Chromosome |
6
show more
close
|
| Expressed Genes |
GFP, Green Fluorescent Protein; birA, biotin ligase), bacteria
show more
close
|
| MGI Accession ID | show more close |
| Site of Expression |
Upon Cre recombination EGFP and birA are expressed in cells where Cre Recombinase is expressed.
show more
close
|
| Strain of Origin |
129
show more
close
|
| Molecular Note |
The targeting vector is designed with, from 5' to 3', a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a floxed-STOP neomycin cassette and polyA sequence, a bacterial birA (biotin ligase) gene, an internal ribosome entry site (IRES), and an enhanced green fluorescence protein (EGFP). This targeting vector was inserted into the Gt(ROSA)26Sor locus.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
show more
close
|
| Breeding Considerations |
When maintaining a live colony, homozygotes may be bred together.
show more
close
|
| Breeding Strategy |
Homozygote x Homozygote
show more
close
|
For Research Use Only.
Related Products