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B6.Cg-Igs7tm211(tetO-ChrimsonR/tdTomato, CAG-tTA2)Tasic/J
Cat. No.: CEMM-07251107
Common Name: TITL-ChrimsonR-tdT-Kv2.1-ICF-IRES-tTA2
Ai211 (also called Ai211(TITL-ChrimsonR-tdT-Kv2.1-ICF-IRES-tTA2)) is a dual-recombinase responsive (cre/flp-dependent), Tet-controllable, fluorescent and optogenetic tool allele created by targeted insertion at the Igs7 locus (TIGRE). Exposure to both Cre and Flp recombinase results in expression of tTA2 and ChrR-tdT (a light-gated cation channel; neuronal activation) in those cells where cre and flp expression overlaps/intersects. ChrR-tdT expression may be expected to be diminished/eliminated by doxycycline.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm211(tetO-ChrimsonR/tdTomato,CAG-tTA2)Tasic
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| Allele Name |
targeted mutation 211
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible; Transactivator; Epitope tag
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The targeting vector contains (from 5' to 3') a Bxb1 attL site, a partial green fluorescent protein sequence (non-functional 500bp fragment to inCrease the space between the Bxb1 attL site and the insulator sequence to improve stability of the targeting vector), two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a Tet response element/promoter (tetO), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), ChrimsonR-tdTomato (ChrR-tdT) sequence with a Kv2.1 sequence at the 3' end followed by a 6xHis tag, T7 tag and XPress tag sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from pCAGGS), a FRT flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), an internal ribosome entry site (IRES) sequence, a synthetic modified tetracycline-regulated transactivator gene (tTA2(S)), a WPRE, a BGH polyA, a PhiC31 attB site, a PGK-5'hygro cassette, an mRNA splice donor, a Bxb1 attR site, SA, 3' hygro-SV40pA, and PhiC31 attP. ChrR-tdT is the result of a ChrimsonR coupled to the fluorescent reporter tdTomato. ChrimsonR (ChrR) is a light-gated cation channel is optimally excited by light of approximately 590nm wavelength (aka, red-shifted). ChrimsonR is an artificial channelrhodopsin derived by introducing the K176R mutation into the alga-derived channelrhodopsin Chrimson.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. no attempts have been made to make this strain homozygous. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice.
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For Research Use Only.
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