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STOCK Gt(ROSA)26Sortm1(Luc)Kael Tg(UBC-CCR5, -CD4)19Mnz/J
Cat. No.: CEMM-07250501
These transgenic mice express human CD4 and CCR5 under the regulatory control of the human ubiquitin C promoter in combination with a widely-expressed luciferase reporter regulated by the Gt(ROSA)26Sor promoter. The mice enable the rapid measurement of HIV-1 transmission by peritoneal and genitomucosal HIV-1 pseudovirus entry in vivo. The system represents a model for the pre-clinical evaluation of prophylactic or vaccine candidates.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(UBC-CCR5,-CD4)19Mnz
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| Allele Name |
transgene insertion 19
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| Allele Attributes |
Inserted expressed sequence; Humanized sequence
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| Gene Symbol |
Tg(UBC-CCR5,-CD4)19Mnz
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| Gene Name |
transgene insertion 19
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| Chromosome |
UN
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| Expressed Genes |
CCR5, C-C motif chemokine receptor 5 (gene/pseudogene), human; CD4, CD4 molecule, human
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| MGI Accession ID | show more close |
| Site of Expression |
Human CD4 and CCR5 are expressed on peripheral blood mononuclear cells.
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| Strain of Origin |
C57BL/6
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| Molecular Note |
A transgene composed of human CCR5 and CD4 on a single poly-protein transcript separated by a ribosomal skip 2A was placed downstream of thehuman ubiquitin C promoter. A linearized 4.5 Kb fragment containing the transgene was injected into C57BL/6J fertilized female pronuclei. Founder line 19 was chosen based on surface expression of hCD4 and hCCR5 on peripheral blood mononuclear cells as determined by flow cytometry.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
FVB/NJ
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| Breeding Considerations |
Animals homozygous for both mutations are viable and fertile.
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For Research Use Only.
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