Online Inquiry
C57BL/6-Gt(ROSA)26Sortm7(Pik3ca*, EGFP)Rsky/J
Cat. No.: CEMM-07250216
Common Name: R26STOPFLP110*
The R26StopFLP110* conditional allele is targeted to the Gt(ROSA)26Sor locus and has a loxP-flanked Neo-STOP cassette preventing transcription of the phosphatidylinositol 3-kinase, catalytic, alpha polypeptide gene (Pik3ca or P110α) and EGFP. These R26StopFLP110* mice allow inducible expression of activated PIK3 heterodimer activity and may be useful in studying signaling cascades that control cell survival, growth, and proliferation, as well as differentiation, regeneration, hypertension and cancer.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm7(Pik3ca*,EGFP)Rsky
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| Allele Name |
targeted mutation 7
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Constitutively active; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of the stop signal results in expression of EGFP and a constitutively active form of P110 in Cre-expressing tissues.
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| Strain of Origin |
C57BL/6
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| Molecular Note |
The P110*-transgene is a targeting vector designed with (from 5' to 3') a splice acceptor site, a loxP-flanked Neo-STOP cassette, a cDNA sequence encoding P110* (a constituitively active form of mouse P110alpha [Pik3ca; the catalytic subunit of phosphatidylinositol 3-kinase]), an FRT flanked internal ribosomal entry site-enhanced green fluorescent protein cassette (IRES-EGFP), and bovine polyadenylation signal. This transgene construct was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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