C57BL/6-Tg(Nes-cre/Esr1*)1Kuan/J

Cat. No.: CEMM-07250250

Common Name: nestin-CreER

The nestin-CreER transgene contains the rat nestin enhancer/hsp68 minimal promoter directing expression of a tamoxifen-inducible Cre recombinase (Cre-ERT1). When these nestin-CreER transgenic mice are bred with mice containing a loxP-flanked sequence, tamoxifen-inducible Cre-mediated recombination is expected to result in deletion of the floxed sequences in the Cre recombinase-expressing tissues of the offspring. These mice may be useful for studying embryonic and adult neurogenesis.

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Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Nes-cre/Esr1*)1Kuan show more close
Allele Name	transgene insertion 1 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Tg(Nes-cre/Esr1*)1Kuan show more close
Gene Name	transgene insertion 1 show more close
Chromosome	UN show more close
Expressed Genes	Cre, Cre recombinase, bacteriophage P1 show more close
MGI Accession ID	4420448 show more close
Site of Expression	Following Tamoxifen administration, Cre recombinase activity is reported in adult neural progenitors in the subventricular zone (SVZ), perinatal SVZ glioblasts, embryonic neural progenitors, and postnatally transformed radial glial cells. show more close
Strain of Origin	C57BL/6 show more close
Molecular Note	The transgene construct contains the rat nestin second intron enhancer fused to an hsp68 (Hspa1b) minimal promoter driving a 1.6 kb Cre sequence fused to the tamoxifen-inducible mutant estrogen receptor (Esr1). The linearized construct was microinjected into pronuclei of fertilized C57BL/6 eggs. Three lines of nestin-Cre/Esr1 mice were generated. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Considerations	When maintaining a live colony, transgene carrier mice may be bred with wildtype (non-carrier) mice from the colony or with C57BL/6J inbred mice. show more close

For Research Use Only.