C57BL/6-Gt(ROSA)26Sor^{tm1(CAG-hVOS1.5)Mbja}/J

Gt(ROSA)26Sor^{tm1(CAG-hVOS1.5)Mbja}

targeted mutation 1

Conditional ready (e.g. floxed); Reporter; No functional change

Gt(ROSA)26Sor

gene trap ROSA 26

6

CFP, Cyan Fluorescent Protein, jellyfish

5911835

Cre recombinase expression in Ai35-hVOS1.5 cells results in bright fluorescence following membrane depolarization (such as neuronal activation) in the Cre-expressing cells.

C57BL/6N-Atm1Brd

The targeting vector is designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), an FRT site, a loxP-flanked STOP cassette (with stop codons in all 3 reading frames and a triple polyA signal), the hVOS1.5 DNA sequence (described below), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a BGH polyA signal and an attB/attP-flanked PGK-FRT Neo-polyA cassette. The entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus. hVOS1.5 is an optimized hybrid voltage sensor consisting of cerulean fluorescent protein (CeFP) with a C-terminal truncated h-ras farnesylation motif that tethers it to the inner face of the plasma membrane.

C57BL/6J C57BL/6NJ

Both heterozygous and homozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together or to wildtype mice from the colony. Alternatively, homozygous mice may be bred together.