STOCK Gt(ROSA)26Sortm1(CAG-mCherry, -EYFP*)Liiz/J

Cat. No.: CEMM-07250916

Common Name: STARS
STARS (STochastic gene Activation with Regulated Sparseness) knock-in mice express mCherry or Cre-dependent membrane bound YFP (meYFP). They are suitable for use in applications related to both CNS and PNS neural circuit mapping and sparse genetic manipulation, as well as visualization of the detailed morphology of other cell types.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm1(CAG-mCherry,-EYFP*)Liiz
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Allele Name
targeted mutation 1
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Allele Attributes
Conditional ready (e.g. floxed); Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
The transgenic construct contains from 5' to 3' an attB site, a chicken beta-actin promoter (with cytomegalovirus immediate early enhancer), a Lox3172 site, mCherry reporter with a polyA tail, a 7.2 kb spacer, a loxP site, Lox3172 site, membrane bound enhanced yellow fluorescent protein (meYFP), woodchuck hepatitis virus posttranscriptional regulatory element and polyadenylation signal (WPRE-pA),a loxP site and an attB site and is integrated into the Gt(ROSA)26Sor locus using PhiC31 integrase RNA.
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HUSBANDRY
Suggested Controls
C57BL/6J
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Breeding Considerations
When maintaining a live colony, these mice can be bred as homozygotes. The Donating Investigator has not tested activity of the mutant allele in homozygotes.
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For Research Use Only.
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