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B6;129-Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat/J
Cat. No.: CEMM-07250432
Common Name: CAG-Sun1/sfGFP
These Sun1-tagged mice are used in the INTACT (isolation of nuclei tagged in specific cell types) method for immunopurification of nuclei. Cre-dependent removal of a floxed STOP cassette allows expression of the SUN1 fusion protein at the inner nuclear membrane in targeted cell types. These nuclei can be immunopurified with antibodies against GFP or MYC for transcriptional and epigenetic studies.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm5(CAG-Sun1/sfGFP)Nat
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| Allele Name |
targeted mutation 5
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inserted expressed sequence
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein; Sun1, Sad1 and UNC84 domain containing 1, mouse, laboratory; Myc, myelocytomatosis oncogene, mouse, laboratory
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| MGI Accession ID | show more close |
| Site of Expression |
The SUN1 fusion protein (SUN1 fused to sfGFP) is expressed at the inner nuclear membrane in targeted cell types where promoters driving Cre recombinase are expressed.
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| Strain of Origin |
129
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| Molecular Note |
A targeting vector containing (from 5' to 3') a CAG promoter, a loxP site flanked STOP cassette, the mouse Sun1 cDNA fused with 2 copies of the superfolder GFP (sfGFP) green fluorescent protein gene and 6 copies of the Myc tag, and an FRT site flanked neomycin resistance gene cassette was inserted into the locus. Removal of the floxed stop cassette is required to achieve expression.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Homozygotes and heterozygotes are viable and fertile.
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| Breeding Strategy |
Homozygote x Homozygote
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For Research Use Only.
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