B6.Cg-Tg(Dmp1-cre/ERT2)D77Pdp/J

Cat. No.: CEMM-07250740

Common Name: 10kb-Dmp1-Cre-ERT2 transgenic line D77

has been discontinued. We recommend B6.Cg-Tg(Dmp1-cre/ERT2)0022Pdp/IkalJ as a replacement.10kb-Dmp1-Cre-ERT2 transgenic line D77 has the 10 kbp promoter/enhancer sequences from the mouse dentin matrix protein 1 locus (DMP1) directing tamoxifen-inducible Cre recombinase (cre/ERT2) expression to osteocytes, some mature osteoblasts and bone lining cells.

Inquiry

Status	Live Mouse Frozen Embryo
Age	4 weeks 12 weeks Customized Age
Sex	Male Female

GENETICS
Allele Symbol	Tg(Dmp1-cre/ERT2)D77Pdp show more close
Allele Name	transgene insertion D77 show more close
Allele Attributes	Recombinase-expressing; Inducible show more close
Gene Symbol	Tg(Dmp1-cre/ERT2)D77Pdp show more close
Gene Name	transgene insertion D77 show more close
Chromosome	UN show more close
Expressed Genes	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene show more close
MGI Accession ID	5297120 show more close
Site of Expression	Tamoxifen-inducible Cre recombinase activity is expressed in osteocytes, some mature osteoblasts and bone lining cells. show more close
Strain of Origin	(C57BL/6 x C3H)F1 show more close
Molecular Note	A fragment (-9624 - +4439) of the Dmp1 gene containing a 9624 bp promoter region, the 95 bp exon 1, 4326 bp intron 1 and 17 bp of the initial non-coding region of exon 2 was cloned into a vector in front of the Cre/ERT2 cDNA. The linearized vector was injected into B6C3F1 pronuclei. 14 pups that were positive for Cre integration were identified and six independent founders were crossed to wild-type B6 mice. Two founders demonstrated penetrant excision of the STOP cassette when crossed to Rosa26-lacZ reporter mice, and were analyzed in greater detail. Two 10kb-Dmp1-Cre-ERT2 transgenic founder lines (D77 and 0022) were identified to have tamoxifen-inducible Cre recombinase activity in both osteocytes and mature osteoblasts. The two lines were similar with the only difference that line D77 had greater expression in mature osteoblasts. show more close

HUSBANDRY
Suggested Controls	C57BL/6J show more close
Breeding Strategy	Homozygote x Homozygote show more close

For Research Use Only.