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B6.Cg-Igs7tm174(tetO-EGFP/RNAi:Hdac1, CAG-tdTomato, -tTA2)Tasic/J

Cat. No.: CEMM-07250976

Common Name: Ai174 [or Ai174(TIT2L-EGFP-shHDAC1-ICL-tdT-tTA2)]
Ai174 (also called Ai174(TIT2L-EGFP-shHDAC1-ICL-tdT-tTA2)) is a Cre-dependent, Tet-controllable histone deacetylation tool strain with dual fluorescent reporters, created by targeted insertion at the Igs7 locus (TIGRE). Exposure to Cre recombinase removes both STOP cassettes, resulting in tTA2 expression, robust tdTomato fluorescence and EGFP/shHDAC1 fusion protein expression (robust EGFP fluorescence and potential knockdown of endogenous histone deacetylase 1 expression by short hairpin RNA shHDAC1). The EGFP/shHDAC1 expression may be expected to be diminished/eliminated by doxycycline.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Igs7tm174(tetO-EGFP/RNAi:Hdac1,CAG-tdTomato,-tTA2)Tasic
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Allele Name
targeted mutation 174
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Allele Attributes
Conditional ready (e.g. floxed); Reporter; Inducible; Transactivator
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Gene Symbol
Igs7
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Gene Name
intergenic site 7
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Chromosome
9
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MGI Accession ID
6514369
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Strain of Origin
(129S6/SvEvTac x C57BL/6NCrl)F1
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Molecular Note
The vector is designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a Tet response element/promoter (TRE2; details below), a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), an EGFP/RNAi:Hdac1 fusion protein (described below), a woodchuck hepatitis virus post-transcriptional regulatory element, a BGH polyA, two copies of chicken beta-globin HS4 insulator element, a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a lox2272-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-TKpA), a tdTomato sequence, a viral 2A oligopeptide), a synthetic modified tetracycline-regulated transactivator gene (tTA2(S)), a WPRE, a BGH polyA, an AttB site, a PGK-5'hygro cassette, an RNA splice donor, a FRT5 site, SA, 3'hygro, SV40pA, and AttP. The TRE2 promoter used here is Tet-responsive P>hCMV*-1min CMVhCMV*-1< is silent in the absence of tTA or rtTA binding to tetO.The EGFP/RNAi:HDAC1 fusion protein is composed of a synthetic enhanced green fluorescent protein sequence (EGFP), an ~150bp linker sequence and a 97bp short hairpin RNA sequence (tgctgttgacagtgagcgagcttgggtaatagcagccatttagtgaagccacagatgtaaatggctgctattacccaagcctgcctactgcctcgga) designed to target/silence the mouse histone deacetylase 1 locus (Hdac1).
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For Research Use Only.
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