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B6;129S-Nos1tm1.1(cre/ERT2)Zjh/J

Cat. No.: CEMM-07250292

Common Name: nNOS-CreER
The nNOS-CreER-KI knock-in allele was designed to both abolish neuronal nitric oxide synthase 1 (Nos1) gene function and direct CreERT2 fusion protein expression to nNOS positive GABAergic neurons by the endogenous Nos1 promoter/enhancer elements. Cre-ERT2 fusion gene activity is inducible by tamoxifen administration.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Nos1tm1.1(cre/ERT2)Zjh
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Allele Name
targeted mutation 1.1
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Allele Attributes
Recombinase-expressing; Inducible
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Gene Symbol
Nos1
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Gene Name
nitric oxide synthase 1
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Chromosome
5
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Expressed Genes
cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene
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MGI Accession ID
4941223
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Site of Expression
Following Tamoxifen administration, Cre recombinase activity is observed in nNos GABAergic neurons.
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Strain of Origin
(C57BL/6 x 129S4/SvJae)F1
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Molecular Note
A targeting vector was designed to insert a CreERT2 fusion gene (Cre-ERT2; Cre recombinase fused to a G400V/M543A/L544A triple mutation of the human estrogen receptor ligand binding domain), an SV40 polyA signal, and an FRT flanked neo cassette into the initiation codon of the neuronal nitric oxide synthase 1 locus (Nos1). This construct was electroporated into C57BL/6;129S-derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with 129S mice to originate the colony. Mutant mice were bred with Actin-FLPe mice to remove the neo selection cassette and the FLPe transgene was subsequently bred out of the line.
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HUSBANDRY
Suggested Controls
B6129SF2/J
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Breeding Considerations
When maintaining a live colony, heterozygous mice may be bred together or to wildtype siblings. The donating investigator has not fully characterized the homozygous phenotype to date.
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Breeding Strategy
Heterozygote x +/+ sibling
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For Research Use Only.
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