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B6;129-Igs1tm1(CAG-Bgeo, -tdTomato/TEVP, -SV2B/GFP)Nat/J
Cat. No.: CEMM-07250184
A targeted insertion placed 2 kb 5' of the Igs1 (intergenic site, formerly Ubb) gene provides constitutive expression of a nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) in the absence of Cre-mediated recombination. Following Cre-mediated recombination, the NLS-beta-geo coding region is excised and a downstream open reading frame is expressed. When Cre-mediated recombination occurs in neurons, the amino terminal piece (tdTomato/TEVP, a 6xmyc epitope, a TEVP cleavage site) labels all axon and dendrites, and the carboxy-terminal portion (SV2B, GFP, 3xHA epitope) labels presynaptic structures. This reporter can be activated by cre recombinase in any cell in the body at any time in development.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs1tm1(CAG-Bgeo,-tdTomato/TEVP,-SV2B/GFP)Nat
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; No functional change
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| Gene Symbol |
Igs1
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| Gene Name |
intergenic site 1
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| Chromosome |
11
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| Expressed Genes |
Bgeo, fusion of beta-galactosidase and neomycin phosphotransferase genes, E. coli; myc tag
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| MGI Accession ID | show more close |
| Site of Expression |
Cre excision of a nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo) results in self-cleavage of the construct. Expression of the amino-terminal, containing tdTomato, and the carboxy-terminal portion, containing EGFP, are reported in Cre-expressing tissues.
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| Strain of Origin |
129
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| Molecular Note |
The following elements were inserted 2 kb 5' of Ubb via homologous recombination: CMV enhancer/beta-actin promoter (CAG), loxP site, nuclear-localized beta-galactosidase/neomycin resistance protein reporter (NLS-beta-geo), 3 stop transcription sites, loxP site, tdTomato/Tobacco etch virus protease (TEVP), a 6xMyc epitope, a TEVP cleavage site, synaptic vesicle protein 2B (SV2B), GFP, 3xHA (hemagluttinin) epitope, rabbit beta-globin 3' UTR.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
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| Breeding Considerations |
When maintained as a live colony, heterozygotes may be bred. Homozygotes are not healthy.
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For Research Use Only.
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