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B6.FVB(Cg)-Tg(Pmch-cre)1Lowl/J
Cat. No.: CEMM-07251062
Common Name: MCH-Cre
MCH-Cre transgenic BAC mice express cre recombinase from the mouse Pmch (pro-melanin-concentrating hormone; also called MCH) promoter/enhancer regions. These mice, backcrossed to C57BL/6J and lacking the Pde6brd1 retinal degeneration allele, are useful in studies of MCH-neuronal activity in controlling energy balance, glucose homeostasis, sleep, locomotion, and reward-related behaviors.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Tg(Pmch-cre)1Lowl
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| Allele Name |
transgene insertion 1
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| Allele Attributes |
Recombinase-expressing
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| Gene Symbol |
Tg(Pmch-cre)1Lowl
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| Gene Name |
transgene insertion 1
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| Chromosome |
UN
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| Expressed Genes |
Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Cre expression is directed specifically to the melanin-concentrating hormone (MCH) neurons of the hypothalamus and zona incerta.
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| Strain of Origin |
FVB/NJ
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| Molecular Note |
Homologous recombination with EL250 cells was induced to insert a Cre-polyA-FRT Neo-FRT cassette into a BAC containing the Pmch (pro-melanin-concentrating hormone; MCH) at the Pmch translational start site with concomitant replacement of the 26 nucleotides following it. Arabinose-induced Flp recombination in bacterial cells removed the kanamycin cassette. The purified, linearized construct was injected into the pronuclei of FVB one-cell embryos. Eight lines were generated and crossed to Z/EG reporter animals. 2 lines exhibited GFP expression in the lateral hypothalamus and zona incerta, known sites of endogenous Pmch expression. Cre expression in one of these lines of characterized further.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
C57BL/6J
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| Breeding Considerations |
Hemizygotes are viable and fertile. Homozygous viability/fertility has not been assessed.
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| Breeding Strategy |
Hemizygote x Noncarrier; Noncarrier x Hemizygote
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For Research Use Only.
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