Online Inquiry
STOCK En1tm8.1Alj/J
Cat. No.: CEMM-07250084
These En1flox mice harbor loxP-flanked homeodomain portion of exon 2 of the engrailed 1 (En1 locus. When bred to mice that express Cre recombinase, the resulting offspring will have this region deleted in the cre-expressing tissue(s). As such, these mice may be useful in generating conditional mutations for studying engrailed protein function in the embryonic mesencephalon and rhombomere 1, as well as developing cerebellum, limbs, somites, and skin.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
En1tm8.1Alj
show more
close
|
| Allele Name |
targeted mutation 8.1
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); No functional change
show more
close
|
| Gene Symbol |
En1
show more
close
|
| Gene Name |
engrailed 1
show more
close
|
| Chromosome |
1
show more
close
|
| MGI Accession ID | show more close |
| Strain of Origin |
129S6/SvEvTac
show more
close
|
| Molecular Note |
A targeting construct was designed to insert an FRT flanked neomycin cassette and loxP site upstream, and a loxP site downstream of the second exon of the targeted gene. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss mice (to avoid rescue of the En1 mutant phenotype by the C57BL/6 background). The resulting En1flox-neo mice were then bred to 129 mice constitutively expressing Flp recombinase to remove the Frt-flanked neo cassette, generating En1flox offspring.
show more
close
|
| HUSBANDRY | |
|---|---|
| Breeding Considerations |
When maintaining a live colony, homozygous mice may be bred.
show more
close
|
For Research Use Only.
Related Products