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B6.Cg-Igs7tm134.1(tetO-COP4*H134R/EYFP)Hze/J
Cat. No.: CEMM-07250858
Common Name: Ai134(TITL-ChR2H134R-EYFP)-D; Ai134D; Ai134(TITL-ChR2-YFP)-D
Ai134(TITL-ChR2H134R-EYFP)-D mice (also called Ai134D or Ai134(TITL-ChR2-YFP)-D) are a Cre/Tet-dependent, optogenetic line - created by targeted insertion at the Igs7 locus (TIGRE; an intergenic region on mouse chromosome 9 that allows reporter expression to be tightly regulated). Following Cre-mediated removal of the STOP cassette, they may be used to generate Tet-Off/Tet-On mutant animals with conditional (inducible/reversible) expression of an improved channelrhodopsin2/EYFP fusion protein (ChR2(H134R)-EYFP). Subsequent illumination of ChR2(H134R)-expressing (EYFP fluorescent) cells with blue light leads to reversible photostimulation of action potential firing/neural activity in these cells.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm134.1(tetO-COP4*H134R/EYFP)Hze
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| Allele Name |
targeted mutation 134.1
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter; Inducible
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| Gene Symbol |
Igs7
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| Gene Name |
intergenic site 7
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| Chromosome |
9
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| Expressed Genes |
COP4, Channelrhodopsin, Chlamydomonas; YFP, Yellow Fluorescent Protein, jellyfish
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| MGI Accession ID | show more close |
| Site of Expression |
When bred with other mice expressing Cre recombinase and tetracycline-controlled transactivator protein (tTA) or reverse tetracycline-controlled transactivator protein (rtTA), ChR2(H134R) expression in cells/tissues where the expression patterns of the individual promoters driving Cre and tTA/rtTA overlap can be regulated with tetracycline or its analog doxycycline (dox).
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The vector is designed with (from 5' to 3') an FRT3 site, two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a Tet response element/promoter, a floxed-STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), the ChR2(H134R)-EYFP fusion sequence, a WPRE (to enhance mRNA stability), a BGH polyA, two copies of chicken beta-globin HS4 insulator element, an AttB site, a PGK-5'hygro cassette, an RNA splice donor and a FRT5 site. PhiC31-mediated recombination removed the AttB/AttP-flanked sequence and replaced it with the recombined AttB/AttP site (AttL).
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For Research Use Only.
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