Online Inquiry
B6;129S6-Igs7tm213(CAG-EGFP, CAG-mOrange2, CAG-mKate2)Tasic/J
Cat. No.: CEMM-07250980
Common Name: Ai213 [or Ai213(TICL-EGFP-ICF-mOrange2-ICN-mKate2)]
Ai213 (also called Ai213(TICL-EGFP-ICF-mOrange2-ICN-mKate2)) is a triple-recombinase responsive reporter allele designed with a CAG promoter, loxP-STOP-loxP and EGFP coding sequence, followed by a CAG promoter, frt-STOP-frt and mOrange2 coding sequence which is followed by a CAG promoter, nox-STOP-nox and mKate2 coding sequence - all inserted into the Igs7 locus (TIGRE). Exposure to either Cre, FLP or Nigri recombinase results in EGFP fluorescence, mOrange2 fluorescence or mKate2 fluorescence, respectively. In total, Ai213 mice are a Cre::FLP::Nigri intersectional fluorescent reporter line designed to allow EGFP/mOrange2/mKate2 fluorescence in those cells where Cre, FLP and Nigri expression overlaps. Ai213 may also be useful as dual-recombinase responsive fluorescent reporter (e.g., Cre/FLP-dependent EGFP/mOrange2, Cre/Nigri-dependent EGFP/mKate2 and/or Cre/Nigri-dependent EGFP/mKate2 fluorescence), as well as a single recombinase responsive fluorescent reporter (e.g., Cre-dependent EGFP, FLP-dependent mOrange2 and/or Nigri-dependent mKate2 fluorescence).
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Igs7tm213(CAG-EGFP,CAG-mOrange2,CAG-mKate2)Tasic
show more
close
|
| Allele Name |
targeted mutation 213
show more
close
|
| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
show more
close
|
| Gene Symbol |
Igs7
show more
close
|
| Gene Name |
intergenic site 7
show more
close
|
| Chromosome |
9
show more
close
|
| MGI Accession ID | show more close |
| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
show more
close
|
| Molecular Note |
The vector contains (from 5' to 3') a Bxb1 attB site, a partial green fluorescent protein sequence (non-functional 500bp fragment to inCrease the space between the Bxb1 attB site and the insulator sequence to improve stability of the targeting vector), two copies of chicken beta-globin HS4 insulator element (to reduce reporter gene expression in absence of transactivators), a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, a loxP-flanked STOP cassette (stop codons in all three reading frames linked to synthetic pA-hGHpA-PGKpA), a synthetic enhanced green fluorescent protein sequence (EGFP), a woodchuck hepatitis virus post-transcriptional regulatory element, a BGH polyA, two copies of chicken beta-globin HS4 insulator element, the CAG promoter, an FRT flanked STOP cassette, a monomeric orange fluorescent protein sequence (mOrange2; details below) followed by 2xHA tag sequence, a WPRE, a BGH polyA, a PhiC31 attB site, a WPRE, a PGK polyA, two copies of chicken beta-globin HS4 insulator element, the CAG promoter, a nox-flanked STOP cassette, a sequence encoding the monomeric far-red fluorescent protein mKate2 (details below), a viral 2A oligopeptide that mediates ribosomal skipping, a WPRE, a BGH polyA, a PhiC31 attB site, a PGK-5'hygro cassette, an RNA splice donor, Bxb1 attR-SA-3'hygro-SV40pA-PhiC31 attP. mOrange2 is a mammalian-codon optimized, photostable, fast-bleaching, monomeric orange fluorescent protein. Compared to its predecessor, mOrange2 has substantially enhanced photostability (Q64H, F99Y, E160K and G196D mutations) and also exhibits slightly shifted excitation and emission peaks (549nm and 565nm, respectively). Kate2 is a monomeric far-red fluorescent protein optimized for high-brightness, photostability, pH resistance and low-toxicity. Compared with its predecessor, the fluorescence of mKate2 is far more pH-stable, thus inCreasing its brightness at physiological pH and making mKate2 a more reliable reporter for imaging in acidic organelles.
show more
close
|
| HUSBANDRY | |
|---|---|
| Suggested Controls |
Wild-type from the colony
show more
close
|
| Breeding Considerations |
Heterozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. To date, it has not been attempted to make this strain homozygous. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to C57BL/6J inbred mice.
show more
close
|
| Breeding Strategy |
Wild-type x Heterozygote; Heterozygote x Wild-type
show more
close
|
For Research Use Only.
Related Products