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BALB/c-Gt(ROSA)26Sortm1(CAG-EGFP, -BphP1/mCherry/TetR)Vvv/J
Cat. No.: CEMM-07251042
Common Name: loxP-BphP1
These loxP-BphP1 mice are a Cre-dependent, Tet-controllable optogenetic line, created by targeted insertion of a CAG promoter, loxP-flanked EGFP-polyA cassette and the photoreceptor/fluorescent/tet-repressor fusion protein (RpBphP1/mCherry/TetR) into the the Gt(ROSA)26Sor locus. Widespread EGFP expression is replaced by RpBphP1/mCherry/TetR fusion protein expression upon Cre recombinase exposure. Further introduction of a BphP1 binding partner::nuclear-localization tag::VP16 activation domain construct and a tetO (TRE) promoter-driven reporter gene allow the subsequent control of reporter gene expression via illumination with near-infrared (NIR) light (~740-780 nm) as well as tetracycline/doxycycline.
Inquiry
| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm1(CAG-EGFP,-BphP1/mCherry/TetR)Vvv
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| Allele Name |
targeted mutation 1
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| Allele Attributes |
Reporter; Inducible
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| MGI Accession ID | show more close |
| Site of Expression |
Widespread EGFP expression before Cre recombinase exposure, and then photoreceptor/fluorescent/tet-repressor fusion protein (RpBphP1/mCherry/TetR) expression after Cre recombinase exposure. RpBphP1 is a purple photosynthetic bacterium Rhodopseudomonas palustris-derived bacteriophytochrome photoreceptor P1. mCherry is a monomeric red fluorescent protein. TetR is the tetracycline (tet) repressor sequence that binds the tetO (TRE) promoter sequences. The RpBphP1/mCherry/TetR fusion protein does not have any nuclear localization signal or VP16 activation domain sequences.
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| Strain of Origin |
BALB/c
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| Molecular Note |
The targeting vector was designed with (from 5' to 3') a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (from pCAGGS), a loxP site, an enhanced green fluorescent protein sequence followed by an SV40 polyA signal, a loxP site, the RpBphP1/mCherry/TetR fusion protein (containing the sequences encoding a purple photosynthetic bacterium Rhodopseudomonas palustris-derived bacteriophytochrome photoreceptor P1 [RpBphP1], the monomeric red fluorescent protein [mCherry] and the tet repressor sequence [TetR]), a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE; to enhance the mRNA transcript stability), a bovine growth hormone polyA sequence (BGHpA) and an FRT flanked neomycin resistance cassette. This entire targeting vector was inserted between exons 1 and 2 of the Gt(ROSA)26Sor locus via electroporation.
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| HUSBANDRY | |
|---|---|
| Suggested Controls |
BALB/cByJ
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| Breeding Considerations |
Heterozygous and homozygous mice are viable and fertile with no reported gross physical or behavioral abnormalities. When maintaining a live colony, heterozygous mice may be bred together, to wildtype mice from the colony or to BALB/cByJ inbred mice. Alternatively, homozygous mice may be bred together.
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| Breeding Strategy |
Heterozygote x Heterozygote
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| Coat Color |
BALB/c genetic background mice are expected to be albino. The expected coat color is white.
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For Research Use Only.
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