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STOCK Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo/J

Cat. No.: CEMM-07250380

Common Name: R26TT
The R26TT allele has a CMV enhancer/chicken beta-actin core promoter-driven "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus on chromosome 6. These R26TT mice exhibit widespread expression of tdTomato-3Myc (tdT3Myc) and represent the tdTomato-expressing control strain for "new MADM-6" (new mosaic analysis with double markers on chromosome 6) experiments.
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Status Live Mouse
Frozen Embryo
Age 4 weeks
12 weeks
Customized Age
Sex Male
Female
GENETICS
Allele Symbol
Gt(ROSA)26Sortm10(ACTB-tdTomato)Luo
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Allele Name
targeted mutation 10
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Allele Attributes
Reporter
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Gene Symbol
Gt(ROSA)26Sor
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Gene Name
gene trap ROSA 26
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Chromosome
6
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Expressed Genes
RFP, Red Fluorescent Protein, coral; myc tag, myc tag
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MGI Accession ID
5314256
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Site of Expression
These mice have widespread tdTomato expression When using the "new MADM-6" system.
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Strain of Origin
(129X1/SvJ x 129S1/Sv)F1-Kitl+
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Molecular Note
Cre-mediated recombination between Gt(ROSA)26Sortm6(ACTB-EGFP*,-tdTomato)Luo and Gt(ROSA)26Sortm7(ACTB-EGFP*)Luo produces this allele. The allele has the CMV enhancer/chicken beta-actin core promoter (pCA) upstream of the recombined "MADM TT" cassette inserted into the Gt(ROSA)26Sor locus. The "MADM TT" cassette has an ATG start codon, a beta-globin intronic sequence (containing a FRT flanked region of two lox site variants [lox2272 and lox5171] followed by a single loxP site), an ATG-less tdTomato sequence (nucleotides 3-1548 including stop codon) tagged with three copies of the Myc epitope at its C-terminus, and an SV40 T-antigen poly(A) signal.
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HUSBANDRY
Breeding Considerations
When maintaining a live colony, homozygous mice may be bred together.
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For Research Use Only.
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