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B6.129P2(129S4)-Hprttm12(Ple177-EGFP/cre)Ems/Mmjax
Cat. No.: CEMM-07250141
These Ple177-EGFPCre;mEMS762 mice have the Ple177-EGFPCre transgene targeted as a single copy "knock-in" into the upstream region of hypoxanthine guanine phosphoribosyl transferase (Hprt) locus on the X chromosome. As the promoter/regulatory regions of the human regulator of G-protein signaling 16 (RGS16) gene direct expression of an EGFPCre fusion protein to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina, these Ple177-EGFPCre;mEMS762 mice may be useful in studying RGS16-expressing cells in the brain and diseases affecting the brain.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Hprttm12(Ple177-EGFP/cre)Ems
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| Allele Name |
targeted mutation 12
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| Allele Attributes |
Recombinase-expressing; Reporter
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| Gene Symbol |
Hprt
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| Gene Name |
hypoxanthine guanine phosphoribosyl transferase
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| Chromosome |
X
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| Expressed Genes |
GFP, Green Fluorescent Protein; Cre, Cre recombinase, bacteriophage P1
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| MGI Accession ID | show more close |
| Site of Expression |
Expression of an EGFPCre fusion protein is directed to hippocampus, thalamus, forebrain, cortex, brainstem, cerebellum, and retina.
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| Strain of Origin |
129P2/OlaHsd
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| Molecular Note |
The Ple177-EGFPCre transgene (pEMS1089) was designed with the 1302 bp Ple177 minipromoter (RGS16-C; derived from an upstream candidate regulatory regions and a subsection of the human regulator of G-protein signaling 16 (/RGS16/) promoter) upstream of a minimal F5 mutant-/frt/ site, an EGFP/Cre fusion protein (enhanced green fluorescent protein (with mutated stop) and Cre recombinase), a nuclear localization signal, a second minimal /frt/ site, an SV40 early polyA signal, and a human HPRT complementary sequence (containing exon1, intron1, exon2, and part of intron2).
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| HUSBANDRY | |
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| Breeding Considerations |
The donating investigator recommends maintaining this strain by breeding heterozygous females with C57BL/6J inbred males.
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For Research Use Only.
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