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B6.Cg-Gt(ROSA)26Sortm3.3(CAG-EGFP, -CHRM3*/mCherry/Htr2a)Pjen/J
Cat. No.: CEMM-07250623
Common Name: RC::L-hM3Dq
RC::L-hM3Dq is a Gq-coupled DREADD allele with a cre-dependent FLEx switch containing an eGFP sequence and an inverted hM3Dq/mCherry/2ACT88 fusion protein. Upon exposure to Cre recombinase, the widespread and robust eGFP fluorescence may be replaced with somatodendritic hM3Dq/mCherry expression. The hM3Dq DREADD induces the canonical Gq pathway (depolarization/activation) specifically following administration of its pharmacologically inert ligand (CNO). The RC::L-hM3Dq allele and its derivatives permit chemogenetic/pharmacogenetic activation defined by Cre recombinase expression.
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| Status | Live Mouse Frozen Embryo |
| Age | 4 weeks 12 weeks Customized Age |
| Sex | Male Female |
| GENETICS | |
|---|---|
| Allele Symbol |
Gt(ROSA)26Sortm3.3(CAG-EGFP,-CHRM3*/mCherry/Htr2a)Pjen
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| Allele Name |
targeted mutation 3.3
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| Allele Attributes |
Conditional ready (e.g. floxed); Reporter
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| Gene Symbol |
Gt(ROSA)26Sor
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| Gene Name |
gene trap ROSA 26
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| Chromosome |
6
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| Expressed Genes |
GFP, Green Fluorescent Protein
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| MGI Accession ID | show more close |
| Site of Expression |
EGFP fluorescence is widely expressed. mCherry fluorescence is expressed in Cre expressing tissues after Cre recombination.
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| Strain of Origin |
(129S6/SvEvTac x C57BL/6NCrl)F1
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| Molecular Note |
The allele has a CAG hybrid promoter,a rox-flanked His3-SV40 transcriptional STOP cassette, an FRT flanked STOP cassette and a variant lox-flanked region containing an eGFP cassette, an inverted hM3Dq/mCherry fusion protein, WPRE, polyA signal and an attB/attP-flanked PGK-FRT Neo-polyA cassette all inserted into the Gt(ROSA)26Sor locus. The inverted hM3Dq-mCherry-2ACT88 fusion protein cDNA sequence contains (hM3Dq DREADD) a red fluorescent protein mCherry and the final 264 coding nucleotides of the rat Htr2A gene (encoding a C-terminal epitope that mediates localization to the cell body/soma and dendrites). PhiC31-mediated recombination removed the AttB/AttP-flanked neo sequence. Dre-mediated recombination removed the rox-flanked STOP cassette. Flp-mediated recombination removed FRT flanked STOP cassette.
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For Research Use Only.
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